We narrowed to 33 results for: human dat

...N222/6R] Clcn4 Human Mouse IgG2a 114475 Anti-PARIS/ZNF746 [N196/16R] PARIS/ZNF746 Human Mouse IgG2a 114476...Mucolipin-3 Human Mouse IgG2a 114478 Anti-GABA(A)R, Epsilon [N413/67R] GABA(A)R, Epsilon Human Mouse IgG2a...Anti-Stonin-2 [N346/9R] Stonin-2 Human Mouse IgG2a 114485 Anti-NSD3 [N348/82R] NSD3 Human Mouse IgG2a 114486 Anti-Flrt3...Menke's disease protein) Human Mouse IgG2a 114503 Anti-LRRK1 [N341/37R] LRRK1 Human Mouse IgG2a 114504 Anti-TrpC4...Anti-Malin [N85/18.1R] Malin Human Mouse IgG2a 114514 Anti-DNMT3L [N117/9.5R] DNMT3L Human Mouse IgG2a 114515 Anti-GABA...N147/6.1R] Pan-QKI Human Mouse IgG2a 114525 Anti-FGF13/FHF2 [N91/27.1R] FGF13/FHF2 Human Mouse IgG2a 114527...1.1R] Neurexin-1-Beta Human Mouse IgG2a 114533 Anti-Laforin [N84/37.1R] Laforin Human Mouse IgG2a 114536 ...

... pFETCh_Donor CEBPB Human FLAG pFETCh_CEBPB PX458_CEBPB_1 PX458_CEBPB_2 AHR Human FLAG pFETCh_AHR PX458...AHR_1 PX458_AHR_2 ARID4B Human FLAG pFETCh_ARID4B PX458_ARID4B_1 BCL6_iso1 Human FLAG pFETCh_BCL6_iso1 PX458..._BCL6_iso1_2 CEBPG Human FLAG pFETCh_CEBPG PX458_CEBPG_1 PX458_CEBPG_2 RAD21 Human FLAG pFETCh_RAD21 PX458...PX458_RAD21 GABPA Human FLAG pFETCh_GABPA PX458_GABPA_1 PX458_GABPA_2 GABPB1_iso2 Human FLAG pFETCh_GABPB1...iso2 PX458_GABPB1_iso2_1 ATF1 Human FLAG pFETCh_ATF1 PX458_ATF1 CEBPA Human FLAG pFETCh_CEBPA PX458_CEBPA...PX458_CEBPA_2 CREB1 Human FLAG pFETCh_CREB1 PX458_CREB_1 PX458_CREB_2 TGIF2 Human FLAG pFETCh_TGIF2 PX458...PX458_TGIF2_2 SSRP1 Human FLAG pFETCh_SSRP1 PX458_SSRP1_1 PX458_SSRP1_2 ZNF146 Human FLAG pFETCh_ZNF146...

...Description Human SLC Activation Library 132561 Superti-Furga CRISPR activation library targeting human SLC proteins...proteins. Human SLC Knockout Library 132552 Superti-Furga CRISPR knockout library targeting human SLC proteins...Inducible barcoded overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 ...) are the largest family of transporters in the human genome with approximately 450 identified members...collection contains codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221... knockout library targeting mouse SLC proteins. Human SLC Overexpression Library 213694 Superti-Furga ...Superti-Furga CRISPR knockout library targeting human transmembrane transporters. Feedback Please send...

... Tagging in Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find ...passaging of human iPS cells (Link opens in a new window) RNP transfection for gene editing human iPS cells...fluorescently tagged proteins of key cellular structures in human cell lines. Depositor... opens in a new window) of fluorescently-tagged human induced pluripotent stem cell (hiPSC) lines. The...fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full description...). Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol ...Institute for Cell Science data portal (Link opens in a new window) , including: data and images from the hiPS...

...elegans ), fruit fly, zebrafish, mouse, rat, and human. Developed by the Stanley Qi Lab . CCTop - CRISPR...location, and genome annotation. Available genomes are human (hg19 & hg38), mouse (mm10), and yeast (strain w303... a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm...on-target activity for selected target(s). Uses human, mouse, and rat reference genomes. Hosted by the Broad...meningitidis Cas9 PAMs. Currently supports: mouse and human. Developed by the George Church Lab . CRISPR Optimal... GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF, 108 KB Fujii iChIP/enChIP to...GABPA, and RAD21 PDF, 138 KB Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector...

...previously observed instance of human serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library...adapted from the synthetic human phosphoproteome. It subdivides the 110,139 human phosphoserine site library...phosphosite library can then be used to identify candidate human substrates of kinases of interest by mass spectrometry...pSerOTS-C1* (V70) (DOCX, 551 KB) . The synthetic human phosphoproteome The Rinehart lab synthesized 110,139.... A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics . Nature...iSPI, rEcoli XPS, pSerOTS-C1* (V70) (DOCX, 551 KB) Human Serine Phosphopeptide Library described in: Barber... Isaacs, F. J., & Rinehart, J. (2018). Encoding human serine phosphopeptides in bacteria for proteome-...

... 2013) Express human DNMT1 with a Myc tag. (Li et al. J Biol Chem. 2006) Express human ATM with Flag and.... (Marquart et al. Nat Methods. 2024) Base edit human mitochondrial DNA using mitoBEs . (Yi et al. Nat...Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons with a CRISPRi-based platform...and efficient generation of oligodendrocytes from human induced pluripotent stem cells. (Ehrlich et al. ... . Find plasmids for endogenous gene tagging in human cells from the Allen Institute for Cell Science ...new window) Michael J Fox Foundation (Link opens in a new window) A foundation dedicated to finding a cure...Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource page ...

... ) Rats ( Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells ( Miller et al., Nat Biotechnol. 2011... ; Reyon and Tsai et al., Nat Biotechnol 2012 ) Human pluripotent stem cells ( Hockemeyer et al., Nat ...Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013 ) More detailed...Endogenous Zebrafish Genes TALENs for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional...REAL-Fast and FLASH, including detailed and up-to-date protocols, can be found on the TALengineering website...

...Developing Human Brain - includes developmental transcriptome information, prenatal LMD microarray data, ISH...ISH image data, and anatomic reference atlases of prenatal and adult human brain (Link opens in a new window...Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (..., suggesting that restoring MECP2 expression in humans could be curative. (Link opens in a new window)...common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats are available from (Link opens...conditional deletion of Xist Unpublished Joost Gribnau Human Cell Line Models Induced pluripotent stem cells ...mammalian or bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations...

...Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN...Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly... for deletion mutagenesis and gene targeting in human and mouse pluripotent stem cells. 48706 pTAL7b Joung... TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein. 49043...in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids...Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove...Engineering Reagents Assembly via serial ligation. Validated in zebrafish somatic cells. Keith Joung Zhang ...

...below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate...These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single...This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected...(Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via...GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing genome-wide knockout screens...smaller Cas9 from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene...These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous...

...editing. These edits can include mutations seen in human patients, protein tags, or loxP/FRT sites, among...germline editing, with the International Summit of Human Gene Editing convened in 2015 to discuss the ethics...plasmids for the following models: Mammalian systems (Human, mouse, and rat) Bacteria ( E. coli , Streptococcus...2013. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . ... Approach for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu...Multicolor CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7...DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human genome engineering via Cas9. Science . 339(6121)...

...p53 is the most frequently mutated gene (>50%) in human cancer; most of these mutations are missense mutations...mutagens like radiation or viruses (e.g., HPV, the human papillomavirus). These mutations interfere with .... Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury...45-53. PubMed PMID: 11780111 . p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B,...activated by cellular stresses like DNA damage, oxidative stress, and nutrient deprivation. p53 is activated...component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2...

...Initiative aims to revolutionize our knowledge of the human brain through the development and application of...cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter Edward Boyden 107712...pAAV-CAG-DIO-ChR2(H134R)-eYFP Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic...pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch...Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch...pAAV-syn-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct...core has 100% seq conservation between mouse and human Jonathan Ting 163509-PHPeB CN1839-rAAV-hSyn1-SYFP2...

...display vector system for producing human antibodies Synthetic Human Antibodies: Selecting and Evolving...Beavil Cloning and mammalian expression of mouse and human IgG monoclonals with reduced immune effector functions...produced in-house and undergo application-specific validation and quality control by Addgene as well as by ...collection — monoclonal antibodies extensively validated for neuroscience research applications from the...

... stalwart research model. With more than 70% of human disease genes having an ortholog in zebrafish, this...has more recently emerged as a model system for human disease and translational research, including the...cancer, inflammation and infection, regeneration, human neurological disorders, microbiota studies, and ...Model Organism Database (ZFIN) - A curated database of genetic, genomic, and developmental data for zebrafish...CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated in zebrafish...expression atlas - A zebrafish-specific gene expression database that provides information about when genes are...active in developing embryos. zfRegeneration - A database for gene expression profiling during regeneration...

...) limiting. This PAM is abundant throughout the human genome, but an NGG sequence may not be positioned...activate or repress gene expression in mice and human cells. The simplest dCas9-based activators and repressors...CRISPRoff maintains gene silencing across much of the human genome following transient expression of the dCas9... mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology , 31 (9), 822–826. PMID...Norville, J. E., & Church, G. M. (2013). RNA-Guided Human Genome Engineering via CAS9. Science , 339 (6121...Yamamoto, T. (2014). Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system...Doudna, J. A. (2014). Enhanced homology-directed human genome engineering by controlled timing of CRISPR...

...endogenously-tagged gene variants or to differentiate human iPSCs into other cell types, as noted in the purpose...cellular background. All cell lines and phenotypic datasets that result from this effort will be broadly shared...

...cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor and tumor suppressor... National Cancer Institute (NCI) NCI Biomarker Database International Cancer Genome Consortium...

...Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For information about each catalog...option is an economical choice that can be used to validate the performance of various serotypes before conducting...