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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27 results for: vent

Showing: 1 - 20 of 27 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...using a new vented falcon tube for every two transformations. Each of the four 14 mL Vented Falcon Tubes...use 350 mL of LB Agar per bioassay plate. 14 mL Vented Falcon Tubes (BD Biosciences, 352059) 3X LB Agar...freezer. Aliquot 3 mL SOC into each of four 14 mL Vented Falcon Tubes and have 1 mL SOC per electroporation...Remove all liquid from cuvette and add to 14 mL vented Falcon Tube containing 3 mL SOC. Repeat for each...transformed DNA-Endura from cuvette. Shake four 14 mL Vented Falcon Tubes at 30-37 ℃, 225 rpm for 1 hour. After...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...HEK293 cells Recombinant antibody plasmid DNA 500 mL vented flask 50 mL conical tubes Microcentrifuge tubes...at a density of 0.9 x 10 6 cells/mL in a 500 mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...and hazards for these reagents. Work in a well-ventilated space and under a fume hood when working with... affects the solubility of the RNA. Pro-Tip To prevent overdrying, watch the pellet and carefully remove...for RNA. Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ... for RNA.Store your RNA sample(s) at -80 °C to prevent RNA degradation and avoid multiple freeze-thaw ...

  4. Weighing Reagents Protocol

    Type
    Protocol
    ...might need to weigh it out in the fume hood to prevent exposure. Read the reagent’s material safety data...used to contain the material you’re weighing and prevent cross-contamination with other substances. They...spatula is clean, and sterile (if necessary), to prevent any potential contamination. Scoop out the material...

  5. Water Bath Protocol

    Type
    Protocol
    ...water bath with distilled water, not tap water, to prevent salts from accumulating on the surfaces of the ...evaporates from the tub. Disinfectant may be added to prevent growth of bacteria or fungi. There are disinfectants...water bath cover on the top of the water bath to prevent evaporation and maintain the desired temperature...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ...Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute developed a novel method for the easy...Biolabs by TelesisBio. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. (2009). Enzymatic assembly... new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis of the...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ...concentration disolved in the appropriate liquid solvent. See table to the right for appropriate antibiotic...bottles. The extra empty volume is necessary to prevent your molten agar from boiling over in the autoclave...psi for at least 30 min. The high pressure will prevent your gel mix from boiling over at high temperature...

  8. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the ...stock on dry ice while streaking onto LB agar will prevent it from thawing completely and will improve the...

  9. Fluorescence Titering Assay

    Type
    Protocol
    ... cells. Titering methods assume one integration event per cell. When the percentage exceeds 40% you risk...risk counting cells with multiple integration events leading to underestimation of the true titer. Calculate...

  10. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...insert will be added in the correct orientation and prevents the vector from ligating to itself during the ...phosphatase removes the 5' phosphate and therefore prevents the ligase from being able to fuse the two ends...

  11. Pipetting Protocol

    Type
    Protocol
    ... needed to prevent contamination. When not in use, the tip box should be closed to prevent contamination...

  12. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...may also exhibit varying numbers of integration events. In other words, if an MOI >1 was used, some cells...rates, especially if that transgene is toxic. Eventually, the rapidly growing low-level transgene expressors...

  13. DNA Quantification

    Type
    Protocol
    ...the background inherent to the machine and your solvent. If using a NanoDrop to measure your samples, place...

  17. General Transfection

    Type
    Protocol
    ... time, allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...

  18. Lentivirus Production

    Type
    Protocol
    ... time, allow them to mix and recheck the pH to prevent over or undershooting the desired pH. Allow the...

  19. Colony Formation Titering Assay

    Type
    Protocol
    ...titers as the likelihood of multiple integration events decreases as the dilution increases. Calculate ...
Showing: 1 - 20 of 27 results