We narrowed to 28 results for: SEC

1. CRISPR Library Amplification

   Type

   Protocol

   ...add 10 µL of the pool to 990 µL LB then perform a second and then third 1:100 dilution). Plate 100 µL of...not present on the most dilute plate, count the second plate (ie. the 1:10,000 dilution). After 12-18 ...

2. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...digestion with EcoRI and AgeI. The oligos from section B contain the shRNA sequence flanked by sequences...polymerase has difficulty reading through the secondary structure of the hairpin sequence. Back to Top...

3. DNA Quantification

   Type

   Protocol

   ...despite the accuracy of the NanoDrop, if two consecutive samples have significantly different concentrations...

4. Protocol - How to Design Primers

   Type

   Protocol

   ... be relatively simple and contain no internal secondary structure to avoid internal folding. One also ...

5. Lab Safety for Biosafety Levels One and Two

   Type

   Protocol

   ...impenetrable by a needle, and must be able to close securely. Biohazard signs should be placed in all areas...

6. Using a Light Microscope Protocol

   Type

   Protocol

   ...the microscope stage. If using a slide, you can secure it into place using the metal clips on the stage...

7. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   .... Figure 3: Example of an SDS-PAGE gel of 10 consecutive gradient fractions followed by silver stain. ...

8. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...well in sparse/individual cultures due to lack of secreted growth factors, so not every cell type will be...