Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 21 - 40 of 42 results

  2. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...sites, will result in similar sized bands, thus making this simple digest less informative. This is particularly...freezer and you are not 100% sure it is what you are looking for, but you have a map and know exactly what it...

  3. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Liquid Bacterial Culture You may also like... Streaking and Isolating Bacteria on an LB Agar Plate Creating...Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require...

  4. Centrifugation

    Type
    Protocol
    ...appropriate for the lab space in which you are working. Even if your samples may not require specific ...centrifuge is clean and that everything appears to be working smoothly. Place your sample tubes into the rotor...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ... with a two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations... fine in an assembly if you want to save time. Working on ice, combine segments in the Gibson Assembly...

  6. Pipetting Protocol

    Type
    Protocol
    ...amounts of liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...

  7. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...syringe and a blunt edge 18 ga Hamilton needle, taking care to avoid bubbles (Figure 1). 8 mL of 15% iodixanol...facing the 40% step. Collect up to 5 mL per tube, taking care to avoid collecting the proteinaceous material...

  8. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Wear a lab coat, eye protection and gloves when working with this chemical. Note: If you add EtBr to your...the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield, gloves...

  9. AAV ddPCR Titration

    Type
    Protocol
    ...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

  10. CRISPR Library Amplification

    Type
    Protocol
    ... 30-37 ℃, 225 rpm for 1 hour. After the 1 hour shaking period, pool and gently mix the four tubes. Perform...transformation efficiency! Ensure that no arcing is taking place during electroporation. Arcing would manifest...

  11. Lentivirus ddPCR Titration

    Type
    Protocol
    ...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers/probe...

  12. DNA Quantification

    Type
    Protocol
    ...DNA is resuspended in, but with no DNA added. 'Blanking' measures the background inherent to the machine...

  13. Protocol - How to Design Primers

    Type
    Protocol
    ...regular nucleotide for broader pairing capabilities. Taking into consideration the information above, primers...

  18. Using a Light Microscope Protocol

    Type
    Protocol
    ...until the edges are just out of view. *Pro-Tip* Looking through a microscope’s eyepiece can take some practice...
Showing: 21 - 40 of 42 results