Zinc Finger Consortium: Modular Assembly Kit
Zinc finger nucleases (ZFNs) are synthetic proteins commonly used in genome engineering that contain an custom-designed zinc finger DNA-binding domain fused to the cleavage domain of the FokI endonuclease. This kit includes 141 zinc finger plasmids encoding different zinc finger modules from the Sangamo, Barbas and Toolgen module sets, and can be used to efficiently assemble zinc finger arrays via modular assembly. Also included in this kit also are 5 plasmids and a strain for the bacterial two-hybrid (B2H) system used to screen zinc finger arrays for activity. To request only the B2H reagents, please see the Zinc Finger Consortium: Modular Assembly Accessory Reagents Kit (#1000000010).
This kit consists of 2 plates, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80° C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.