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TALEN Kits Available from Addgene

Addgene is a global, non-profit plasmid repository dedicated to making it easier for scientists to share.

TALENs are important new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease, TALENs bind and cleave DNA in pairs. Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. These collections and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays.

TALEN Kits:

TALEN Kit
Description
Depositing PI
Golden Gate TALEN 2.0
Golden Gate cloning method. Validated in multiple organisms.

Dan Voytas

Adam Bogdanove

Joung Lab TAL Effector Engineering Reagents
Assembly via serial ligation. Validated in zebrafish somatic cells.
Keith Joung
Zhang Lab TALE Toolbox
PCR/Golden Gate cloning method. Optimized for human expression.
Feng Zhang
LIC TAL Effector Assembly Kit
Assembly by ligation-independent cloning (LIC). Validated in human cells.
Veit Hornung

Additional Plasmids for Use with Golden Gate TALEN 2.0:

For Use With:
Plasmid(s)
Depositing PI
Golden Gate TALEN 2.0
RCIscript-GoldyTALEN and pC-GoldyTALEN
Daniel Carlson
Golden Gate TALEN 2.0
TAL5-BB and pTAL6-BB
Tom Ellis
Golden Gate TALEN 2.0
pCS2TAL3-DD and pCS2TALE3-RR
David Grunwald
Golden Gate TALEN 2.0
pCAG-T7-TALEN(Sangamo)-Destination Constructs
Pawel Pelczar
Golden Gate TALEN 2.0
TALEN Construction and Evaluation Accessory Pack
Takashi Yamamoto


Request Plasmids

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