A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5' exonucleolytic degradation.
Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, Weichenrieder O, Coles M, Izaurralde E
Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 10.1038/nsmb.2413. Epub 2012 Nov 11.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66596 | pLNHA-C1-HsXRN1 | expresses lambdaN-HA-tagged XRN1 in mammalian cells |
| 145901 | pAc5.1B-EGFP-DmDCP1_1-127_A | Insect Expression of DmDCP1_1-127 |
| 145902 | pAc5.1B-EGFP-DmDCP1_128-372_A | Insect Expression of DmDCP1_128-372 |
| 145903 | pAc5.1B-lambdaN-HA-DmDCP1_1-127_A | Insect Expression of DmDCP1_1-127 |
| 145904 | pAc5.1B-lambdaN-HA-DmDCP1_128-372_A | Insect Expression of DmDCP1_128-372 |
| 146703 | pAc5.1A-DmXRN1_1-573-V5His6_J | Insect Expression of DmXRN1_1-573 |
| 146704 | pAc5.1A-DmXRN1_1-1141-V5His6_J | Insect Expression of DmXRN1_1-1141 |
| 146705 | pAc5.1A-DmXRN1_574-1141-V5His6_J | Insect Expression of DmXRN1_574-1141 |
| 146706 | pAc5.1A-DmXRN1_574-1612-V5His6_J | Insect Expression of DmXRN1_574-1612 |
| 146707 | pAc5.1A-DmXRN1_1142-1612-V5His6_J | Insect Expression of DmXRN1_1142-1612 |
| 146708 | pAc5.1A-DmXRN1-V5His6_J | Insect Expression of DmXRN1 |
| 146778 | pLNHA-C1-HsXRN1_1174-1342_K | Mammalian Expression of HsXRN1_1174-1342 |
| 146779 | pLNHA-C1-HsXRN1_1174-1458_K | Mammalian Expression of HsXRN1_1174-1458 |
| 146780 | pLNHA-C1-HsXRN1_1174-1706_K | Mammalian Expression of HsXRN1_1174-1706 |
| 146781 | pLNHA-C1-HsXRN1_1343-1534_K | Mammalian Expression of HsXRN1_1343-1534 |
| 146782 | pLNHA-C1-HsXRN1_1535-1706_K | Mammalian Expression of HsXRN1_1535-1706 |
| 146783 | pRSFDuetG-HsEDC4_977-1401_K | Bacterial Expression of HsEDC4_977-1401 |
| 146784 | pT7-EGFP-C1-HsXRN1_K | Mammalian Expression of HsXRN1 |
| 146785 | pT7-EGFP-C1-HsXRN1_1-1173_K | Mammalian Expression of HsXRN1_1-1173 |
| 146786 | pT7-EGFP-C1-HsXRN1_1174-1342_K | Mammalian Expression of HsXRN1_1174-1342 |
| 146787 | pT7-EGFP-C1-HsXRN1_1174-1458_K | Mammalian Expression of HsXRN1_1174-1458 |
| 146788 | pT7-EGFP-C1-HsXRN1_1174-1706_K | Mammalian Expression of HsXRN1_1174-1706 |
| 146789 | pT7-EGFP-C1-HsXRN1_1343-1534_K | Mammalian Expression of HsXRN1_1343-1534 |
| 146790 | pT7-EGFP-C1-HsXRN1_1459-1706_K | Mammalian Expression of HsXRN1_1459-1706 |
| 146791 | pT7-EGFP-C1-HsXRN1_1535-1706_K | Mammalian Expression of HsXRN1_1535-1706 |
| 147050 | pnEA-NvG-DmDCP1_1-127_N | Bacterial Expression of DmDCP1_1-127 |
| 147051 | pnYC-NvHN-DmXRN1_1312-1383_N | Bacterial Expression of DmXRN1_1312-1383 |
| 147052 | pnYC-NvHN-DmXRN1_1323-1355_N | Bacterial Expression of DmXRN1_1323-1355 |
| 147053 | pnYC-NvHN-DmXRN1_1323-1383_N | Bacterial Expression of DmXRN1_1323-1383 |
| 147054 | pRSFDuetG-DmXRN1_1312-1355-DmDCP1_1-127_N | Bacterial Expression of DmXRN1_1312-1355-DmDCP1_1-127 |
| 147055 | pRSFDuetG-DmXRN1_1323-1355-DmDCP1_1-127_N | Bacterial Expression of DmXRN1_1323-1355-DmDCP1_1-127 |
| 147056 | pRSFDuetG-DmXRN1_1323-1383-DmDCP1_1-127_N | Bacterial Expression of DmXRN1_1323-1383-DmDCP1_1-127 |
| 147205 | pAc5.1A-DmXRN1_3xPmut-dsRNAres-V5His6_P | Insect Expression of DmXRN1-3xPmut-dsRNAres |
| 147206 | pAc5.1A-DmXRN1-A1349W-dsRNAres-V5His6_P | Insect Expression of DmXRN1-A1349W-dsRNAres |
| 147207 | pAc5.1A-DmXRN1-M1335AL1338A-dsRNAres-V5His6_P | Insect Expression of DmXRN1-M1335AL1338A-dsRNAres |
| 147208 | pAc5.1A-DmXRN1-P1339A-dsRNAres-V5His6_P | Insect Expression of DmXRN1-P1339A-dsRNAres |
| 147209 | pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P | Insect Expression of DmXRN1-P1339AP1342A-dsRNAres |
| 147210 | pAc5.1A-DmXRN1-Q1346AE1348A-dsRNAres-V5His6_P | Insect Expression of DmXRN1-Q1346AE1348A-dsRNAres |
| 147211 | pAc5.1A-DmXRN1-Q1346AQ1350A-dsRNAres-V5His6_P | Insect Expression of DmXRN1-Q1346AQ1350A-dsRNAres |
| 147212 | pAc5.1A-DmXRN1-Q1346P-dsRNAres-V5His6_P | Insect Expression of DmXRN1-Q1346P-dsRNAres |
| 147213 | pAc5.1A-DmXRN1-W1345A-dsRNAres-V5His6_P | Insect Expression of DmXRN1-W1345A-dsRNAres |
| 147214 | pAc5.1B-EGFP-DmDCP1-Y34A_P | Insect Expression of DmDCP1-Y34A |
| 147215 | pAc5.1B-HA-DmDCP1-N41AS100A-dsRNAres_P | Insect Expression of DmDCP1-N41AS100A-dsRNAres |
| 147216 | pAc5.1B-HA-DmDCP1-Y34A-dsRNAres_P | Insect Expression of DmDCP1-Y34A-dsRNAres |
| 147217 | pAc5.1B-HA-DmDCP1-Y34F-dsRNAres_P | Insect Expression of DmDCP1-Y34F-dsRNAres |
| 147218 | pCIneo-lambdaN-HA-HsDCP1a-Y36A_P | Mammalian Expression of HsDCP1a-Y36A |
| 147219 | pnEA-NpG-HsXRN1_1650-1706_P | Bacterial Expression of HsXRN1_1650-1706 |
| 147220 | pRSFDuetG-DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127_P | Bacterial Expression of DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127 |
| 147221 | pT7-EGFP-C1-HsDCP1a-Y36A_P | Mammalian Expression of HsDCP1a-Y36A |
| 147222 | pT7-EGFP-C1-HsXRN1_1675-1706_P | Mammalian Expression of HsXRN1_1675-1706 |
| 147223 | pETM-60-HsNot3_656-753_P | Bacterial Expression of HsNot3_656-753 |
| 147224 | pnYC-NpHM-HsNot2_429-527_P | Bacterial Expression of HsNot2_429-527 |
| 147225 | pnYC-NpHM-HsNot2_429-540_P | Bacterial Expression of HsNot2_429-540 |
| 147782 | pCIneo-lambdaN-HA-HsEDC4_1-538_V | Mammalian Expression of HsEDC4_1-538 |
| 147783 | pCIneo-lambdaN-HA-HsEDC4_532-979_V | Mammalian Expression of HsEDC4_532-979 |
| 147784 | pCIneo-lambdaN-HA-HsEDC4_974-1265_V | Mammalian Expression of HsEDC4_974-1265 |
| 147785 | pCIneo-lambdaN-HA-HsEDC4_1266-1401_V | Mammalian Expression of HsEDC4_1266-1401 |
| 147786 | pCIneo-lambdaN-HA-HsEDC4-del974-1265_V | Mammalian Expression of HsEDC4-del974-1265 |
| 148285 | pT7-EGFP-C1-HsEDC4_1-538_AA | Mammalian Expression of HsEDC4_1-538 |