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LENTICRISPR-TPI1_sgRNA1
Plasmid#201626PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTPI1 (TPI1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-TPI1_sgRNA2
Plasmid#201627PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertTPI1 (TPI1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPTK004-2-pTPI1
Plasmid#84963PurposeType 2, pTPI1, Triose phosphate isomerase 1 promoterDepositorInsertTriose phosphate isomerase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD-pTPI1 (01-11)
Plasmid#101687PurposepTPI1 in domestication vectorDepositorInsertGGAG-[promoter of S. cerevisiae YDR050C (918bp)]-CCAT
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PTPI1-Dsred-URA3
Plasmid#207017PurposeExpresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).DepositorInsertDsRed-Express 2
UseTagsExpressionYeastMutationPromoterpTPI1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppgk1-PYC-Ttpi1
Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Synthetic, Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpgk1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pPTK004-2-pTPI1
Plasmid#84963PurposeType 2, pTPI1, Triose phosphate isomerase 1 promoterDepositorInsertTriose phosphate isomerase 1 promoter
UseSynthetic BiologyTagsExpressionYeastMutationPromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD-pTPI1 (01-11)
Plasmid#101687PurposepTPI1 in domestication vectorDepositorInsertGGAG-[promoter of S. cerevisiae YDR050C (918bp)]-CCAT
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTPI1-EMC1-GFP-APEX2-URA3
Plasmid#184776PurposeExpresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).DepositorInsertAPEX2
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Emc1-GFP-APEX2-Ura
Plasmid#207074PurposeExpressing Emc1-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertEmc1
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Cox4-GFP-APEX2-Ura
Plasmid#207075PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI
Plasmid#50723Purposeoverexpression of human TPI1 in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI
Plasmid#50719Purposeexpression of human TPI1 in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Lys13Arg
Plasmid#50726Purposeoverexpression of the human TPI1 Lys13Arg allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Lysine 13 to ArgeninePromoterT7Available sinceFeb. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Val
Plasmid#50724Purposeoverexpression of the human TPI1 Ile170Val allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ValinePromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI
Plasmid#50723Purposeoverexpression of human TPI1 in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI
Plasmid#50719Purposeexpression of human TPI1 in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Lys13Arg
Plasmid#50726Purposeoverexpression of the human TPI1 Lys13Arg allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Lysine 13 to ArgeninePromoterT7Available sinceFeb. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Val
Plasmid#50724Purposeoverexpression of the human TPI1 Ile170Val allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ValinePromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET20b- hTPI Ile170Thr
Plasmid#50725Purposeoverexpression of the human TPI1 Ile170Thr allele in E.coliDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTags6xHIS tagExpressionBacterialMutationchanged Isoleucine 170 to ThreoninePromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406-GFP-SNC1(L96V)
Plasmid#105278PurposeIntegrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.DepositorInsertSnc1 (SNC1 Budding Yeast)
UseTagsGFPExpressionYeastMutationLeucine 96 changed to Valine, intron removedPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Ile170Val
Plasmid#50720Purposeexpression of the human TPI Ile170Val allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationIsoleucine 170 to ValinePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-T|C-iGFP-RCY1
Plasmid#105281PurposeOverexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.DepositorInsertRcy1 (RCY1 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Ile170Thr
Plasmid#50721Purposeexpression of the human TPI Ile170Thr allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationIsoleucine 170 to ThreoninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-T|C-iGFP-PH(OSH1)
Plasmid#105280PurposeOverexpresses iGFP-PH(Osh1) under the TPI1 promoter. Integrating at TRP1.DepositorInsertPH domain of Osh1 (SWH1 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterTPI1Available sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Lys13Arg
Plasmid#50722Purposeexpression of the human TPI Lys13Arg allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationchanged Lysine 13 to ArgeninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406-GFP-SNC1(L96V)
Plasmid#105278PurposeIntegrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.DepositorInsertSnc1 (SNC1 Budding Yeast)
UseTagsGFPExpressionYeastMutationLeucine 96 changed to Valine, intron removedPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Ile170Val
Plasmid#50720Purposeexpression of the human TPI Ile170Val allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationIsoleucine 170 to ValinePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only
