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We narrowed to 2,492 results for: MEA

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Showing: 1 - 40 of 2492 results

  7. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    U1a promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  10. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100
    Insert
    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114111-plasmid-map-sequence-id-252509

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  14. p(+)MV-NSE-FlagP-M502-3p

    Plasmid
    #58748
    Purpose
    Expresses recombinant measles virus with Flag-P gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag
    Promoter
    T7
    Available Since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    58748-plasmid-map-sequence-id-90880

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  15. p(+)MV-NSE-FlagP-F497A-3p

    Plasmid
    #58796
    Purpose
    Expresses recombinant measles virus with Flag-P-F497A gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag
    Mutation
    MeV-P F497A
    Promoter
    T7
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58796-plasmid-map-sequence-id-91091

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  16. p(+)MV-NSE-FlagP-F497D-3p

    Plasmid
    #58797
    Purpose
    Expresses recombinant measles virus with Flag-P-F497D gene tagged si2 (siP) target sequence
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag
    Mutation
    MeV-P F497D
    Promoter
    T7
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58797-plasmid-map-sequence-id-91092

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  17. p(+)MV-NSE-FlagrP-3g_haP-F497A-3p

    Plasmid
    #58799
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497A gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag and Ha
    Promoter
    T7
    Available Since
    Aug. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    58799-plasmid-map-sequence-id-91094

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  18. p(+)MV-NSE-FlagrP-3g_haP-M502-3p

    Plasmid
    #58798
    Purpose
    Expresses recombinant measles virus with duplicated P gene tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag and Ha
    Promoter
    T7
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    58798-plasmid-map-sequence-id-91093

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  19. p(+)MV-NSE-FlagrP-3g_haP-F497D-3p

    Plasmid
    #58800
    Purpose
    Expresses recombinant measles virus with duplicated P gene. wt P gene and P-F497D gene are tagged with Flag and HA peptides and si1 (siGFP) and si2 (siP) target sequences, respectively.
    Depositor
    Branka Horvat
    Insert
    Measles virus antigenome
    Tags
    Flag and Ha
    Promoter
    T7
    Available Since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    58800-plasmid-map-sequence-id-91095

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  23. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  26. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Available Since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  28. pDBD-M

    Plasmid
    #50572
    Purpose
    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    Expression
    Yeast
    Promoter
    Gal1
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pDBD-R

    Plasmid
    #50571
    Purpose
    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572
    Depositor
    Alexander Varshavsky
    Article
    Turner et al Science. 2000 Sep 22;289(5487):2117-20.
    Insert
    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    Expression
    Yeast
    Mutation
    M1R (B-gal)
    Promoter
    Gal1
    Available Since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. pCL27-decoder

    Plasmid
    #159449
    Purpose
    Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1tet-citrine-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    Pfus1tet
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159449-plasmid-map-sequence-id-313197

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  36. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    PlexAtet
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159450-plasmid-map-sequence-id-313198

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  37. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    Pfus1mut
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159453-plasmid-map-sequence-id-313208

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Showing: 1 - 40 of 2492 results