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Showing: 1 - 50 of 471 results

  1. pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

    Plasmid
    #105200
    Purpose
    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)
    Depositor
    Timothy Lu
    Article
    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.
    Insert
    G8p-antiPD1(HC)-P2A-antiPD1(LC)
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    105200-plasmid-map-sequence-id-201271

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  2. pD1sE_SC12

    Plasmid
    #219718
    Purpose
    DV1 E dimer with fusion loop mutation and increased expression
    Depositor
    Brian Kuhlman
    Article
    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi
    Insert
    DV1 sE SC12
    Use
    Tags
    8xHis and human serum albumin (HSA)
    Expression
    Mammalian
    Mutation
    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…
    Promoter
    Available since
    May 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    219718-plasmid-map-sequence-id-433425

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  3. pPD176.36

    Plasmid
    #1720
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    T7p, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, lacIN…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1720-plasmid-map-sequence-id-326

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  6. pPD117.20

    Plasmid
    #1712
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc37-448, unc37Tsa, gfpN, IVSR, gfpF64L, gfpS65T…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1712-plasmid-map-sequence-id-318

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  7. pPD158.87

    Plasmid
    #1709
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    sur5-10k, sur5tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1709-plasmid-map-sequence-id-315

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  8. pPD152.79

    Plasmid
    #1704
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    dpy30Tsa, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, …
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1704-plasmid-map-sequence-id-310

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  11. pPD138.11

    Plasmid
    #1721
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    EGFPN, EGFPC, IVSL, myo3-2341, myo3Tsa
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1721-plasmid-map-sequence-id-327

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  12. pPD158.117

    Plasmid
    #1711
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc119tsa, RFPN, RFPivs2, RFPV105A, RFPivs4, RFPS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1711-plasmid-map-sequence-id-317

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  13. pPD10_46

    Plasmid
    #1432
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    OriF1<<, OriF1>>, lacZN, unc54N, unc5…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1432-plasmid-map-sequence-id-43

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  14. pPD157.63

    Plasmid
    #1710
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    vit2tsa, T20G511N, T20G511C, let8583', decoy
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1710-plasmid-map-sequence-id-316

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  15. pPD18_49

    Plasmid
    #1436
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, myo3N, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1436-plasmid-map-sequence-id-47

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  16. pPD158.114

    Plasmid
    #1715
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, IVSA, RFPN, RFPivs2, RFPV105A…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1715-plasmid-map-sequence-id-321

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  17. pPD18_32

    Plasmid
    #1435
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1435-plasmid-map-sequence-id-46

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  18. pPD187.24

    Plasmid
    #1723
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    pie1tsa, SPGFPN, SPGFPC, pie13', T3p, OriF1&…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1723-plasmid-map-sequence-id-329

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  19. pPD157.60

    Plasmid
    #1705
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc25-19k, unc255', T7p, gfpN, IVSR, gfpS65C…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1705-plasmid-map-sequence-id-311

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  20. pPD187.49

    Plasmid
    #1722
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    T7p, SPGFPN, SPGFPC, Sp6p, OriF1>>, OriF1&l…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1722-plasmid-map-sequence-id-328

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  21. pPD16_43

    Plasmid
    #1433
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1433-plasmid-map-sequence-id-44

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  22. pPD159.88

    Plasmid
    #1707
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    rol6tsa, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, I…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1707-plasmid-map-sequence-id-313

    Enlarge

  23. pPD191.45

    Plasmid
    #1708
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    col10-33k, col10tsa, IVSA, gfpN, IVSR, gfpS65C, I…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1708-plasmid-map-sequence-id-314

    Enlarge

  24. pPD172.01

    Plasmid
    #1719
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    sur5-10k, sur5tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1719-plasmid-map-sequence-id-325

    Enlarge

  25. pPD177.66

    Plasmid
    #1717
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1717-plasmid-map-sequence-id-323

    Enlarge

  26. pPD159.62

    Plasmid
    #1714
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, IVSA, RFPN, RFPivs2, RFPV105A…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1714-plasmid-map-sequence-id-320

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  27. pPD177.53

    Plasmid
    #1702
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1702-plasmid-map-sequence-id-308

    Enlarge

  28. pPD177.62

    Plasmid
    #1701
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    pes10UAS-270, pes10Tsa, IVSA, SV40NLS, TetRN, Tet…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1701-plasmid-map-sequence-id-307

    Enlarge

  29. pPD157.99

    Plasmid
    #1713
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, T7p, gfpN, IVSR, gfpS65C, IVS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1713-plasmid-map-sequence-id-319

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  30. GPD1_SB

    Plasmid
    #224147
    Purpose
    IPTG-inducible expression of C. elegans protein GPD-1 with a chitin tag
    Depositor
    Katherine Walstrom
    Insert
    GPD-1 (gpd-1 Nematode)
    Use
    Tags
    Ala-intein-CBD
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224147-plasmid-map-sequence-id-444366

    Enlarge

  35. pPD1cR

    Plasmid
    #86970
    Purpose
    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, G108C in PCNA1
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86970-plasmid-map-sequence-id-173927

    Enlarge

  36. pPD1R

    Plasmid
    #86969
    Purpose
    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH
    Promoter
    T7 promoter
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86969-plasmid-map-sequence-id-173876

    Enlarge

  37. GPD1L

    Plasmid
    #39084
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPD1L (GPD1L Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. GPD1L

    Plasmid
    #38843
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GPD1L (Gpd1l Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pPD16_51

    Plasmid
    #1434
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1434-plasmid-map-sequence-id-45

    Enlarge

  40. pPD107.09

    Plasmid
    #1703
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SupTrp5', SupTrp3', T7p, lacZN, OriF1&g…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1703-plasmid-map-sequence-id-309

    Enlarge

  41. pPD160.60

    Plasmid
    #1706
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc30-29k, unc305', T7p, gfpN, IVSR, gfpS65C…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1706-plasmid-map-sequence-id-312

    Enlarge

  42. pPD176.86

    Plasmid
    #1716
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1716-plasmid-map-sequence-id-322

    Enlarge

  43. pPD177.71

    Plasmid
    #1718
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1718-plasmid-map-sequence-id-324

    Enlarge

  44. pPD132.49

    Plasmid
    #1724
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only

  49. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

    Enlarge

  50. pLV V5-Mospd1

    Plasmid
    #175132
    Purpose
    Lentiviral expression of V5-tagged mouse Mospd1
    Depositor
    Larry Gerace
    Insert
    Mospd1 (Mospd1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175132-plasmid-map-sequence-id-352019

    Enlarge

Showing: 1 - 50 of 471 results