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Plasmid#138589PurposeT7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
UseSynthetic BiologyTagsOmpA export signal and T7 tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trx_dAChE4
Plasmid#83917PurposeEncodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coliDepositorInsertDesigned hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
UseTagsHis, S-tag, and TRXExpressionBacterialMutationV13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…PromoterT7 promoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30a_HIV-RT
Plasmid#153311PurposeBacterial expression of HIV reverse transcriptaseDepositorInsertHIV Reverse Transcriptase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET3aTr
Plasmid#64008PurposeSingle donor or transfer plasmid for source of translation cassette for plasmid suiteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trxAChEwt
Plasmid#83916PurposeEncodes an E. coli codon optimized version of wild type human acetylcholinesteraseDepositorInsertShort form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
UseTagsHis, S-tag, and TRXExpressionBacterialMutationcodon optimised for E.coli expressionPromoterT7 promoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30ax
Plasmid#85761PurposeA bacterial expression vector with a His-tag at N and C-terminus.DepositorTypeEmpty backboneUseTagsHis tag (N-term and C-term)ExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_v2
Plasmid#183692PurposeBacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)DepositorInsertER Halo(K73T)
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET303_sumocodo_GFP_codoopti
Plasmid#210393PurposeExpress SUMO-GFP in E. coliDepositorInsertGFP
UseTagsSUMO-tagExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30_Halotag_K73T_Jdomain
Plasmid#183693PurposeBacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domainDepositorInsertER Halo(K73T)-J domain
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7+-SrtA
Plasmid#105602Purposeexpressed 7+ sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7+ mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET3a-hUBA1
Plasmid#63571PurposeExpression of human UBA1 tagged with HisDepositorInsertUBA1 (UBA1 Human)
UseTagsExpressionBacterialMutationIsoform 2 (1018 amino acids)PromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GAPDH
Plasmid#83910Purposestable overexpressionDepositorInsertglyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30a-HS-Tn5
Plasmid#127916PurposeProkaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.DepositorInsertHyperstable Tn5
UseTagsE coli elongation factor Ts and Mxe intein - Chit…ExpressionBacterialMutationE54K, L372PPromoterT7Available sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet30b-5M SrtA
Plasmid#51140Purposeexpression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 5M
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30-R5-GFP
Plasmid#17838DepositorInsertpET30-GFP
UseSynthetic BiologyTagsHis, R5, and S-tagExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pet30b-7M SrtA
Plasmid#51141Purposeexpression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 7M
UseTags6x HisExpressionBacterialMutationP94R, E105K, E108Q, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET32a-TRXtag
Plasmid#11516DepositorInsertNone - thioredoxin is vector encoded (trxA )
UseTagsHis, His (out of frame), and TrxExpressionBacterialMutationvector modified; removed N-terminal His tag by di…PromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only