We narrowed to 2,372 results for: PUC19

1. pUC19_HR-BirA-FLAG_Ago2

   Plasmid

   #170918

   Purpose

   Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   BirA-FLAG
   

   Expression

   Mammalian

   Promoter

   lac

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19_HR-BirA-FLAG_Ago1

   Plasmid

   #170919

   Purpose

   Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

   Depositor

   Constance Ciaudo

   Article

   Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

   Insert

   BirA-FLAG
   

   Expression

   Mammalian

   Promoter

   lac

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-SpRYgest_targetplasmid (KAC833)

   Plasmid

   #181748

   Purpose

   pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Type

   Empty backbone

   Use

   Substrate plasmid

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-POLBHR-eGFP

   Plasmid

   #176064

   Purpose

   Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   EGFP-PolB (POLB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   pUC with endogenous SapI mutated out, PAM mutatio…

   Promoter

   N/A

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-PpSEC13-mEGFP

   Plasmid

   #160469

   Purpose

   P. pastoris integration vector for the gene replacement of SEC13 fused to mEGFP

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   ppSEC13-mEGFP
   

   Expression

   Yeast

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-PpSEC13-msGFP2

   Plasmid

   #160470

   Purpose

   P. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   ppSEC13-msGFP2
   

   Expression

   Yeast

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-hsg(G:H)

   Plasmid

   #138259

   Purpose

   Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   mCherry targeting sgRNAs G and H
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-hsg(NT:NT)

   Plasmid

   #138260

   Purpose

   Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. puC19 preACT1(WTdel6)

   Plasmid

   #111324

   Purpose

   for T7 transcription of wt actin pre-mRNA (del6)

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Expression

   Bacterial

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. puC19 preACT1(AcAc)

    Plasmid

    #111327

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Expression

    Bacterial

    Mutation

    3' splice site AcAc mutation in intron

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. puC19 preACT1(BrG)

    Plasmid

    #111326

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Expression

    Bacterial

    Mutation

    branch site A to G mutation in intron

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. puC19 preACT1(BrC)

    Plasmid

    #111325

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Expression

    Bacterial

    Mutation

    branch site A to C mutation in intron

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19 gene 60 to gap

    Plasmid

    #49158

    Purpose

    Template for generating gene 60 mRNA, sequence 5' of coding gap only

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Mutation

    Coding gap and downstream sequence have been del…

    Promoter

    T7

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19 - T7 pro - IRES - EGFP

    Plasmid

    #138586

    Purpose

    Express EGFP with an upstream T7 promoter in pUC19 backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    EGFP
    

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid

    #228253

    Purpose

    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19_msfGFP-EWSR1-repair-tempate

    Plasmid

    #237682

    Purpose

    For knock-in msfGFP to EWSR1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-link-mCherry-FLAG:cat

    Plasmid

    #211831

    Purpose

    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mCherry-FLAG-cat
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19_msfGFP-SRRM2-repair-template

    Plasmid

    #238247

    Purpose

    For knock-in msfGFP to SRRM2 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    SRRM2
    

    Use

    CRISPR

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-link-sfGFP-FLAG:Kan

    Plasmid

    #211833

    Purpose

    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    sfGFP-FLAG-Kan
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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