We narrowed to 2,048 results for: SP6

1. pCS2+ H2B-mEmerald

   Plasmid

   #99269

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mEmerald in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mEmerald
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR178

   Plasmid

   #240641

   Purpose

   Template for in vitro transcription of mScarlet-I mRNA using SP6 polymerase.

   Depositor

   John Pringle

   Insert

   mScarlet-I
   

   Use

   Vertebrate gene expression and general in vitro t…

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+ H2B-TagRFP-T

   Plasmid

   #99271

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein TagRFP-T in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   TagRFP-T
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR182

   Plasmid

   #240642

   Purpose

   Template for in vitro transcription of AmNeonGreen mRNA using SP6 polymerase.

   Depositor

   John Pringle

   Insert

   AmNeonGreen
   

   Use

   Vertebrate gene expression and general in vitro t…

   Tags

   Expression

   Mutation

   Codon-optimized for Exaiptasia diaphana, amino ac…

   Promoter

   SP6

   Available since

   Aug. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTLNX

   Plasmid

   #49032

   Purpose

   FX cloning Xenopus oocyte expression vector with SP6 promoter

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Xenopus oocytes expression

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+hSpCas9

   Plasmid

   #51815

   Purpose

   Encode hSpCas9 for in vitro transcription with Sp6

   Depositor

   Masato Kinoshita

   Article

   Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

   Insert

   humanized S.pyogenes Cas9
   

   Use

   CRISPR; Medaka, zebrafish

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTLNXA7

   Plasmid

   #49033

   Purpose

   FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Xenopus oocytes expression

   Tags

   chicken alpha7 signal sequence

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR241

   Plasmid

   #240644

   Purpose

   Expression of AmNeonGreen-13myc in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase

   Depositor

   John Pringle

   Inserts

   AIPGENE865 promoter
   SP6 promoter
   AmNeonGreen
   

   Use

   Gene expression and genomic integration in fish

   Tags

   13myc

   Expression

   Mutation

   Codon-optimized for Exaiptasia diaphana, amino ac…

   Promoter

   Available since

   Aug. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEPQD0KN0285

   Plasmid

   #162290

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPQD0KN0284

    Plasmid

    #162289

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPQD0CB0026

    Plasmid

    #162285

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0CB0246

    Plasmid

    #162286

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0KN0283

    Plasmid

    #162288

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0KN0282

    Plasmid

    #162287

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmtb-t7-alpha-bungarotoxin

    Plasmid

    #69542

    Purpose

    For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

    Depositor

    Sean Megason

    Article

    Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Tol2, beta-actin promoter, sp6,t7 for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    beta-actin, sp6, t7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+ H2B-mCherry

    Plasmid

    #99265

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Article

    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

    Insert

    mCherry
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcRII-mogat3a in situ probe

    Plasmid

    #232135

    Purpose

    zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat3a
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCR197

    Plasmid

    #240643

    Purpose

    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase

    Depositor

    John Pringle

    Inserts

    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    

    Use

    Gene expression and genomic integration in fish

    Tags

    NES (from Exaiptasia diaphana MEK2), NLS (from SV…

    Expression

    Mutation

    Codon-optimized for Exaiptasia diaphana, amino ac…

    Promoter

    Available since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRII-TOPO-dgat1b in situ probe

    Plasmid

    #232138

    Purpose

    zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1b
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6/T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOO2-GW

    Plasmid

    #135097

    Purpose

    Gateway vector for expression of proteins in Xenopus oocytes

    Depositor

    Wolf Frommer

    Article

    Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

    Type

    Empty backbone

    Use

    Bacterial expression vector for generating sp6 tr…

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge