Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 15,186 results for: egf

Clear keywords
Showing: 1981 - 2000 of 15186 results

  1. pEGFP(C3)-Nup153-C (880-1475)

    Plasmid
    #64317
    Purpose
    GFP-tagged mammalian expression of C-terminal domain of Nup153
    Depositor
    Birthe Fahrenkrog
    Publication
    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nup153 (NUP153 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    C-terminal subunit (880-1475)
    Promoter
    CMV
    Available since
    April 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  2. pIRES2-EGFP-deltaNp53:WTp53

    Plasmid
    #49244
    Purpose
    expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells
    Depositor
    Dylan Taatjes
    Publication
    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p53 deltaN and p53 WT (TP53 Human)
    Tags
    His and IRES-EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    49244-plasmid-map-sequence-id-72827

    Enlarge

  3. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid
    #171383
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized minidCas13X.1-REPAIRv2
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    171383-plasmid-map-sequence-id-337350

    Enlarge

  4. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

    Enlarge

  5. Str-STIM1-NN_ST-SBP-EGFP

    Plasmid
    #65262
    Purpose
    synchronize trafficking of ST from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 43 of ST
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65262-plasmid-map-sequence-id-120697

    Enlarge

  7. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid kit
    #1000000236
    Purpose
    Collection of MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly.
    Depositor
    Daniel Voytas
    Application
    Cloning and Synthetic Biology
    Vector type
    Plant Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid
    #123615
    Purpose
    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE3(R33A/K34A)-P2A-EGFP
    Expression
    Mammalian
    Mutation
    R33A/K34A in rAPOBEC1, D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    123615-plasmid-map-sequence-id-239343

    Enlarge

  14. MXS Chaining Kit

    Plasmid kit
    #1000000064
    Purpose
    A collection of standardized building blocks used to assemble plasmids that are optimized for fluorescence imaging or flow cytometry applications.
    Depositor
    Pierre Neveu
    Application
    Cloning and Synthetic Biology
    Vector type
    Mammalian Expression
    Cloning type
    Restriction Enzyme
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid
    #79650
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Publication
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Expression
    Mammalian
    Mutation
    isoleucine 679 changed to lysine
    Promoter
    CMV
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    79650-plasmid-map-sequence-id-156910

    Enlarge

Showing: 1981 - 2000 of 15186 results