We narrowed to 5,083 results for: mcs

1. peGFP-HDAC1 (pc2447)

   Plasmid

   #240148

   Purpose

   Expression vector containing a fusion protein of eGFP and human HDAC1.

   Depositor

   Cristina Cardoso

   Article

   Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12

   Insert

   HDAC1 (HDAC1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. FASTHDR-Cterm-HaloTag-Puromycin

   Plasmid

   #167208

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FASTHDR-Cterm-mClover3-Puromycin

   Plasmid

   #167205

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mClover3

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiTet-P2A-mCherry BlastR

   Plasmid

   #186743

   Purpose

   Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   P2A-mcherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

   Plasmid

   #176395

   Purpose

   Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

   Plasmid

   #163024

   Purpose

   Expression of CRISPRi with U6-gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 polyA, and myc NLS

   Expression

   Mammalian

   Promoter

   hEF-1a

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER21-puro_TAOK3

   Plasmid

   #209778

   Purpose

   doxycycline-inducible expression of TAOK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   TAOK3 (TAOK3 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGFP-Kif5c

   Plasmid

   #71853

   Purpose

   mammalian expression of GFP-Kif5c

   Depositor

   Michelle Peckham

   Article

   Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

   Insert

   Kif5c (Kif5c Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Kif5c starts at AA3

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaM-6

   Plasmid

   #172771

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-6
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-EGFP-Cdc42-wt

    Plasmid

    #12975

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. dSpyCas9-p300-P2A-CASANOVA

    Plasmid

    #113129

    Purpose

    Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    dCas9-p300-P2A-CASANOVA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MIC60-mCherry

    Plasmid

    #232789

    Purpose

    Encodes an mCherry-tagged MIC60/IMMT

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    MICOS complex subunit MIC60 (IMMT Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrc99S-ssDsbA-CRM197-4xDQNAT

    Plasmid

    #128403

    Purpose

    Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HSC1-HS4-D4Z4c-GiP

    Plasmid

    #58541

    Purpose

    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Human EF1-alpha

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

    Plasmid

    #194278

    Purpose

    Expresses gRNA and dSaCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

    Inserts

    humanized dSaCas9 KRAB T2A Thy1.1
    gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    hU6 and hUbC

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 2xFLAG-2xSTREP E2F7

    Plasmid

    #236442

    Purpose

    transient overexpression of E2F7 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F7 (E2F7 Human)
    

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. IL1RN gRNA4

    Plasmid

    #113133

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. IL1RN gRNA2

    Plasmid

    #113131

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PHB2 S39D-mCherry

    Plasmid

    #232787

    Purpose

    Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    PHB2 (PHB2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Serine 39 to Aspartate

    Promoter

    CMV

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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