We narrowed to 13,416 results for: sequence
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Plasmid#177726PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
TagsGFP11, HA, and Mitochondrial Targeting SequenceExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJRoC30_VP8H
Plasmid#188062PurposePROSS-designed versatile peroxidase 8HDepositorInsertPROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PrimeRoot.v2C-FLP
Plasmid#201740PurposeFor DNA insertion test in protoplastDepositorInsertePPE,FLP
UseCRISPRExpressionPlantAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SPI1_P2A_Hygro_Barcode
Plasmid#120482PurposeBarcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCerulean-VSVG
Plasmid#11913DepositorInsertVSVG
ExpressionMammalianMutationF64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 K720A
Plasmid#19939DepositorInsertcullin 1 K720A (CUL1 Human)
TagsHA2ExpressionMammalianMutationCUL1 with a NEDD8 modification mutantAvailable SinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-EGFP
Plasmid#175827PurposeExpress EGFP with microtubule-targeting sequence in mammalian cellsDepositorInsertEGFP with microtuble-targeting sequence
Tagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLACTE-PseudoHT51
Plasmid#99257PurposeContains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresisDepositorInsertFMR1 (FMR1 Human, Synthetic)
MutationContains synthetic, non-human, primer sequence do…Available SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)
Plasmid#193049PurposeEncodes G-418 resistanceDepositorInsertprsp-27::NeoR::unc-54 3’ UTR
ExpressionWormPromoterprsp-27Available SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL758
Plasmid#219504PurposeExpress Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequencesDepositorInsertmsr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
ExpressionMammalianPromoterH1Available SinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-BirA*-HA
Plasmid#214474PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and Lyn11 (Plasma Membrane Localization Se…ExpressionMammalianMutationR118GPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript II KS(+) EACMCV DNA-A 1.6mer
Plasmid#159136PurposeInfectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-ADepositorInsertEACMCV DNA-A 1.6mer
UseInfectious clone for bombarding plantsAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MITF_P2A_Hygro_Barcode
Plasmid#120460PurposeBarcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTDH-His-bio
Plasmid#52331PurposeExpresses full-length Metastasis adhesion protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertMTDH (MTDH Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3−3xFLAG−AGO2(D669A)
Plasmid#220461PurposeExpress catalytically-dead mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
Tags3xFLAGExpressionMammalianMutationAspartate 669 to alaninePromoterCMVAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 Y42A/M43A
Plasmid#19940DepositorInsertcullin 1 Y42A/M43A (CUL1 Human)
TagsHA2ExpressionMammalianMutationCUL1 with a SKP1 binding mutantAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
SERPINC1-bio-His
Plasmid#52076PurposeExpresses full-length Antithrombin-III precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSERPINC1 (SERPINC1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD58-bio-His
Plasmid#53153PurposeExpresses full-length CD58 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD58 (CD58 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 2.1 (VF2468 ZFN target) Ef1a
Plasmid#31483DepositorInsertTraffic Light Reporter 2.1 (V2468 zfn target) EF Puro
UseLentiviralMutationTraffic Light 2.0 series have a +3 PEST sequence …Available SinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only