161,697 results

1. NMD2

   Plasmid

   #216220

   Purpose

   Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns

   Depositor

   Rebecca Voorhees

   Article

   Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

   Insert

   GFP P2A RFP_b-globin intron x2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPdX

   Plasmid

   #85084

   Purpose

   Expresses putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Cysteine 73 to Serine and Cysteine 85 to …

   Promoter

   T7 promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-VHL-pRc/CMV

   Plasmid

   #19999

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQCH

   Plasmid

   #164160

   Purpose

   IncQ BHR plasmid

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

   Plasmid

   #192066

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for Mouse Ctip2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mStrawberry-N1

   Plasmid

   #54644

   Purpose

   Localization: N1 Cloning Vector, Excitation: 574, Emission: 596

   Depositor

   Nathan Shaner, Roger Tsien, Michael Davidson

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (AAV8)

   Viral Prep

   #137148-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP (#137148). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP plasmid DNA. nEF-driven, Cre and Flp-dependent expression of Arch3.3-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Cre and Flp-dependent)

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pLV puro Tet VSVG

   Plasmid

   #82724

   Purpose

   Lentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoter

   Depositor

   Warren Zipfel

   Insert

   VSVG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSico

   Plasmid

   #11578

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF1108

    Plasmid

    #143254

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREBBP (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge