163,421 results

1. pVD1 tRNA-gRNA position E2 (GB2239)

   Plasmid

   #160561

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tRNA-gRNA position E2 (Multiplexing Edit)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Septin 11 isoform II-EGFP

   Plasmid

   #120316

   Purpose

   Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNeptune2-Tubulin-6

   Plasmid

   #56149

   Purpose

   Localization: Microtubules, Excitation: 600, Emission: 650

   Depositor

   Michael Davidson

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mNeptune2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SmBiT-b4GALT1 Fusion Vector

   Plasmid

   #237043

   Purpose

   Express SmBiT-b4GALT1 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   b4GALT1 (B4GALT1 Human)
   

   Use

   Tags

   SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA5/FRT/TO-3xFLAG-His6-LARP1

   Plasmid

   #233626

   Purpose

   Expression of Flag- and His-tagged LARP1

   Depositor

   Shintaro Iwasaki

   Article

   Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

   Insert

   LARP1 (LARP1 Human)
   

   Use

   Tags

   3FLAG-His6

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

   Plasmid

   #223137

   Purpose

   pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

   Plasmid

   #228252

   Purpose

   pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   SpCas9 P23H sgRNA, mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

   Plasmid

   #228253

   Purpose

   pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5/FRT_CMV_Kinprola_PKA-mEGFP-MTBD

   Plasmid

   #233363

   Purpose

   CMV driven expression of the PKA activity recorder Kinprola_PKA in mammalian cell lines fused to mEGFP, targeting to the microtubule

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   Kinprola_PKA-mEGFP-MTBD
   

   Use

   Tags

   mEGFP-MTBD

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR4_miniFXN_8

    Plasmid

    #234361

    Purpose

    Mini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.

    Depositor

    Marek Napierala

    Article

    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

    Insert

    FXN (FXN Human)
    

    Use

    Cloning

    Tags

    Flag Tag

    Expression

    Mutation

    There is a partial deletion in the 5'UTR of …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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