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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,350 results for: 11

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Showing: 2001 - 2020 of 8350 results

  7. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Publication
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  9. pcDNA3-mCherry-LKB1(KD)

    Plasmid
    #84640
    Purpose
    mCherry-tagged, LKB1(K78I); kinase-dead mutant
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-LBK1(K78I) (STK11 Synthetic, Human)
    Tags
    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Mutation
    Lysine 78 mutated to Isoleucine; kinase dead
    Promoter
    CMV
    Available since
    May 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    84640-plasmid-map-sequence-id-176148

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  10. pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid
    #101102
    Purpose
    Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether
    Depositor
    Tudor Fulga
    Publication
    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TMt-dCas9(C)-VP64
    Use
    CRISPR and Synthetic Biology
    Tags
    HA and Myc
    Expression
    Mammalian
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101102-plasmid-map-sequence-id-197199

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  11. pEntry Tshld(FKBP F36V)-GFP

    Plasmid
    #53260
    Purpose
    pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.
    Depositor
    Matthew Pratt
    Publication
    Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tshld(FKBP F36V)-GFP
    Tags
    EGFP, Flag, and HA
    Mutation
    FKBP F36V
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    53260-plasmid-map-sequence-id-82981

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  13. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid
    #70666
    Purpose
    Reduced the expression of gamma-tubulin in murine cells
    Depositor
    Maria Alvarado-Kristensson
    Publication
    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1
    Insert
    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    Use
    U6 promoter
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70666-plasmid-map-sequence-id-133021

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  15. pT7-agrBD-I

    Plasmid
    #53439
    Purpose
    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53439-plasmid-map-sequence-id-83233

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  19. EGFP-Rab7a-7

    Plasmid
    #56443
    Purpose
    Localization: Ras GTPase, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Publication
    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab7a (RAB7A Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
Showing: 2001 - 2020 of 8350 results