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pCFJ1663

Plasmid

#51484

Purpose

miniMos transposable element with HygroR and peel-1, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-PPO-Venus

Plasmid

#139504

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV5 and AAV9

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD8bM-3

Plasmid

#40080

Depositor

Paula Kavathas

Article

Thakral et al J Immunol. 2008 Jun 1;180(11):7431-42.

Insert

CD8bM-3

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
pST39-Hrs-STAM1 core

Plasmid

#21497

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pST39- Hrs(398-514)-His/STAM1(301-387) (STAM, HGS Human)

Tags

His

Expression

Bacterial

Mutation

none

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/FFT-LL5BIP

Plasmid

#112830

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Tags

2xFlag-TEV

Expression

Mammalian

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pOSIP-KH-RBS2-dCas9

Plasmid

#182740

Purpose

Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Insert

dCas9

Use

CRISPR; Integrative vector

Promoter

Ptet

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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f1H (264)

Plasmid

#24425

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

22aa N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
ATF4 13: 5’ATF4.uORF2AUA.GFP

Plasmid

#21860

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF2 was converted to AUA

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pPro7(E)-gfp

Plasmid

#17812

Depositor

Jay Keasling

Article

Lee et al Gene. 2006 Aug 1;377:6-11. doi: 10.1016/

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

Plasmid

#190162

Purpose

Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

Depositor

Samie Jaffrey

Article

Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

Insert

mcherry_(3'UTR, No Actin)_24Broccoli

Tags

24×Broccoli

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only
pHSG-PCNA1

Plasmid

#66125

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 1 (SSO_RS01995 Sulfolobus solfataricus P2)

Use

Cloning vector

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-nsyb-MKIIK11A::nlsLexADBDo

Plasmid

#64726

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKIIK11A::nlsLexADBDo

Expression

Insect

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattB-MKII::nlsLexADBDo

Plasmid

#64718

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKII::nlsLexADBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-nsyb-MKII::GAL4DBDo

Plasmid

#64721

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKIIK11A::GAL4DBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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ACA

Plasmid

#27790

Depositor

Steven Vogel

Article

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Amber-Cerulean-Amber

Use

Stoichiometry standard

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
(attB) tdMCP-tagged BRaf with MS2-circRNA

Plasmid

#226163

Purpose

Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

3xFlag-tdMCP-BRaf (BRAF Human)
IRES-mCherry-P2A-PuroR
MS2-circRNA barcode

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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HaloTag-LaminA

Plasmid

#231894

Purpose

Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

LaminA (LMNA Human)

Tags

HaloTag

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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emiRFP670-cGAS

Plasmid

#231896

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Tags

emiRFP670

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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