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pCHKU38.2-2.2

Plasmid

#110670

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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DDT Wnt

Plasmid

#248843

Purpose

Double Death Trap (DDT) Wnt reporter Containing FKBP12(F36V)-ΔCaspase9 (FC) and Puromycin Resistance Gene for Assessing Wnt Signaling

Depositor

Hai Jiang

Article

He et al Cell Chem Biol. 2025 Oct 22:S2451-9456(2

Insert

7xTCF binding element

Use

Lentiviral

Promoter

7xTCF binding element & minimal TATA-box prom…

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-cOpn5-T2A-mcherry

Plasmid

#237857

Purpose

The transfer plasmid for packaging AAV expressing chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of a EF1α promoter.

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV

Promoter

EF1α

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-cOpn5-T2A-mcherry

Plasmid

#237860

Purpose

The transfer plasmid for packaging AAV expressing chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of GfaABC1D promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV

Promoter

GfaABC1D

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_023d sgCiPELO #1

Plasmid

#228932

Purpose

Expression of dCas9-KRAB and sgRNA targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA355_sgCiCh2-2

Plasmid

#229021

Purpose

Expression of a CRISPRi doxycycline inducible control sgRNA that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_023d sgCiPELO #8

Plasmid

#228934

Purpose

Expression of dCas9-KRAB and sgRNA targeting PELO

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

PELO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_051d_sgCiChr2-2

Plasmid

#228940

Purpose

Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr3

Plasmid

#193660

Purpose

Expression of tandem pre-sgRNA array hcr3 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-CFTR-sgRNA-KLF4-sgRNA-TET1-sgRNA-tRNA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-hcr5

Plasmid

#193661

Purpose

Expression of tandem pre-sgRNA array hcr5 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA-pcr4

Plasmid

#193662

Purpose

Expression of tandem pre-sgRNA array pcr4 for LbCas12a

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-AID

Plasmid

#193647

Purpose

Expression of base editor dCas12a-AID in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hAID, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-BE

Plasmid

#193648

Purpose

Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-rAPOBEC1, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-CDA1

Plasmid

#193649

Purpose

Expression of base editor dCas12a-CDA1 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-PmCDA1, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-eAID

Plasmid

#193650

Purpose

Expression of base editor dCas12a-eAID in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-eAID, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAdEasy-hALDOA D34S

Plasmid

#210813

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Tags

HA

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA D34S

Plasmid

#210807

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET Duet1 His6 SUMO D14

Plasmid

#177854

Purpose

Bacterial Expression of D14

Depositor

Karsten Melcher

Article

Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

Insert

D14

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET24a His6 GST PYL2(14-188)

Plasmid

#177842

Purpose

Bacterial Expression of PYL2

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 2 (PYL2 Mustard Weed)

Tags

6xHis-GST

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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PLKO.1-Cd155 shRNA2

Plasmid

#166488

Purpose

Mouse Cd155 RNAi

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

shRNA for mouse Cd155 (Pvr Mouse)

Use

Lentiviral

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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