We narrowed to 6,916 results for: cre encoding plasmid

1. pAAV-EFIa-DIO-LMO7 (mNeonGreen-eKL9h-VChR1)

   Plasmid

   #205098

   Purpose

   Cre-dependent fusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 (luminopsin LMO7) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Bjorefeldt et al bioRxiv. 2023 Dec 4:2023.06.26.545546. d

   Insert

   mNeonGreen, eKL9h, and Volvox channelrhodopsin-1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

   Plasmid

   #98932

   Purpose

   Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

   Plasmid

   #98931

   Purpose

   Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-DIO-GCaMP6s

   Plasmid

   #166606

   Purpose

   Encodes Cre-dependent GCaMP6s under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS2113

   Plasmid

   #49138

   Purpose

   AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   ssAAV-Ple67-emGFP-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FEV

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEMS2112

   Plasmid

   #49137

   Purpose

   AAV plasmid with NR2E1 (Ple264) promoter driving expression of EmGFP.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   ssAAV-Ple264-emGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   NR2E1

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEMS2114

   Plasmid

   #49139

   Purpose

   AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   ssAAV-Ple67-emGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FEV

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. NXPE1_pcDNA3.4-TOPO

   Plasmid

   #226841

   Purpose

   Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.

   Depositor

   Bert Vogelstein

   Insert

   NXPE1 (NXPE1 Human)
   

   Use

   Tags

   x6 HIS and an IL2 secretion signal both N termina…

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcGFP-N1_CBPdIDR7dAL

   Plasmid

   #221914

   Purpose

   Transient expression of GFP-tagged CBPdeltaIDR7deltaAL in mammalian cells

   Depositor

   Daniel Bose

   Insert

   CBP (CREBBP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deletion of IDR7 (aa 2109-2434); deletion of the …

   Promoter

   Available since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcGFP-N1_CBPdIDR6dAL

    Plasmid

    #221912

    Purpose

    Transient expression of GFP-tagged CBPdeltaIDR6deltaAL in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBP (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of IDR6 (aa 1856-2090); deletion of the …

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMbd3EosHaloTAF_Hyg

    Plasmid

    #140958

    Purpose

    Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    methyl-CpG binding protein 3 (Mbd3 Mouse, Synthetic)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV Ef1a-EGFP-WPRE

    Plasmid

    #135428

    Purpose

    Can be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequence

    Depositor

    Rylan Larsen

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcGFP-N1_CBPKTG

    Plasmid

    #221911

    Purpose

    Transient expression of GFP-tagged CBPKTG in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBP (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    mutation of lysine residues to glycine residues (…

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP-N1_CBPdCFIDdAL

    Plasmid

    #221916

    Purpose

    Transient expression of GFP-tagged CBPdeltaCFIDdeltaAL in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBP (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of the CBP-FUS interaction domain (CFID)…

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pChd4EosHaloTAF_Puro

    Plasmid

    #172099

    Purpose

    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid

    #64218

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E1B

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid

    #64222

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

    Plasmid

    #64220

    Purpose

    Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid

    #64217

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, H1, and U6

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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