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pET30ax

Plasmid

#85761

Purpose

A bacterial expression vector with a His-tag at N and C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

His tag (N-term and C-term)

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCM130

Plasmid

#45828

Depositor

Mary Lidstrom

Article

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Low-background broad-host-range promoter-probe ve…

Promoter

none

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMYT096

Plasmid

#198867

Purpose

Transient CRISPR/Cas9 Vector without a selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGPI-SceI

Plasmid

#32060

Purpose

Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Type

Empty backbone

Use

Mutagenesis plasmid

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEV-G1-Km

Plasmid

#46813

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and PGK1

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMyC

Plasmid

#42192

Depositor

Annabel Parret, Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

ColE1

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pMetOYC-Dest

Plasmid

#105082

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

Depositor

Christopher Grefen

Article

Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

Type

Empty backbone

Tags

Cub-PLV and mOST4

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pU6d:sgRNA#5

Plasmid

#64249

Purpose

expresses sgRNA under U6d promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pUS250

Plasmid

#198322

Purpose

Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein

Depositor

Nicholas Coleman, Mark Somerville

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

cumate-inducible

Available Since

April 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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