We narrowed to 15,649 results for: NSI

1. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of AA 1 to 45

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FLAG-LoxP-GFP-LoxP-SNAP-TERT

   Plasmid

   #71391

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Expression

   Mutation

   Promoter

   Endogenous TERT promotor

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. His-MBP human SPOP (aa 28-337)

   Plasmid

   #52294

   Purpose

   bacterial expression of human SPOP (aa 28-337) fused to His-MBP

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   SPOP (SPOP Human)
   

   Use

   Tags

   His-MBP and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pg-ODD-EGFP

   Plasmid

   #87211

   Purpose

   HIF-1a's ODD domain fused to EGFP

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   HIF-1a ODD domain (HIF1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   ODD domain (AA 530-652)

   Promoter

   CMV

   Available since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenSyn2-EGFP-­OMP25C

   Plasmid

   #71427

   Purpose

   Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.

   Depositor

   Henri Huttunen

   Article

   Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi:

   Insert

   OMP25 (172-­‐206) (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 172-206 only

   Promoter

   synapsin

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3/hArf6(WT)-HA

   Plasmid

   #79424

   Purpose

   Expresses C-terminally HA-tagged Arf6(WT) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_BTK-1/1

   Plasmid

   #91350

   Purpose

   Protein expression and purification of human SH3 domain construct BTK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   BTK-1/1 (BTK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_ITK-1/1

   Plasmid

   #91253

   Purpose

   Protein expression and purification of human SH3 domain construct ITK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ITK-1/1 (ITK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP Cep170 (Nigg PID200)

   Plasmid

   #41150

   Depositor

   Erich Nigg

   Article

   Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

   Insert

   Cep170 (CEP170 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOTTC385 - pAAV CMV-IE IRES EGFP

    Plasmid

    #102936

    Purpose

    An AAV vector that expresses IRES EGFP under the CMV-IE promoter

    Depositor

    Brandon Harvey

    Article

    Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMA7-SacB

    Plasmid

    #79968

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    Levansucrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP SNAP47

    Plasmid

    #101915

    Purpose

    Expresses SNAP47 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP47 (SNAP47 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3/hArf5(WT)-mCherry

    Plasmid

    #79421

    Purpose

    Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX302_HOXB13

    Plasmid

    #70089

    Purpose

    Expression of human HOXB13 from CMV promoter, puromycin selection marker

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET32a-HD16Q

    Plasmid

    #11487

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 16 glutamines (HTT Human)
    

    Use

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 16 glutamines, vector m…

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pHH0103_GRB2-1/2

    Plasmid

    #91480

    Purpose

    Protein expression and purification of human SH3 domain construct GRB2-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    GRB2-1/2 (GRB2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiPGK Puro DEST PKAKTRClover

    Plasmid

    #59153

    Purpose

    Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA (PRKACA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-FLEX-ArchT-tdTomato

    Plasmid

    #28305

    Purpose

    Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Has Service

    AAV5

    Insert

    ArchT-tdTomato
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LentiRCas9-CUG

    Plasmid

    #104183

    Purpose

    Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

    Depositor

    Eugene Yeo

    Article

    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

    Insert

    U6-CUGsgRNA, EFS-PIN-dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-FLEX-ArchT-GFP

    Plasmid

    #28307

    Purpose

    Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 7TFP CDH1 mutant reporter

    Plasmid

    #91703

    Purpose

    lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    CDH1 mutant promoter (CDH1 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    mutant E-boxes

    Promoter

    CDH1 mutant

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid

    #107292

    Purpose

    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-GFP (SNCA Human)
    

    Use

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHES839

    Plasmid

    #87941

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pLL8 lenti-D10Acas9-3xflag-blast

    Plasmid

    #112134

    Purpose

    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    D10Acas9-3xflag-blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A mutant in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHH0103_FYN-1/1

    Plasmid

    #91413

    Purpose

    Protein expression and purification of human SH3 domain construct FYN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FYN-1/1 (FYN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP VAMP7 (1-120)

    Plasmid

    #42317

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Longin domain (1-120) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Δ120-220

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

30. eGFP-PRM-5R

    Plasmid

    #112155

    Purpose

    Mammalian expression plasmid for PRM-5R in a vector expressing eGFP.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    PRM-5R (ABL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains only the PRM domain of Abl1

    Promoter

    CMV

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLX_TRC311 ETV1

    Plasmid

    #74981

    Purpose

    Constitutive ETV1 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHH0103_BCAR1-1/1

    Plasmid

    #91418

    Purpose

    Protein expression and purification of human SH3 domain construct BCAR1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BCAR1-1/1 (BCAR1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PXPR007 sgCIC-2

    Plasmid

    #74959

    Purpose

    Cas9 + sgCIC-2 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PXPR007 sgCIC-1

    Plasmid

    #74953

    Purpose

    Cas9 + sgCIC-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    CIC (CIC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHES795

    Plasmid

    #87943

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Synthetic, Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA5/FRT/TO-AT1R-APEX

    Plasmid

    #96847

    Purpose

    For expression of AT1R-APEX in mammalian cells

    Depositor

    Andrew Kruse

    Article

    Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

    Insert

    AT1R-APEX (AGTR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. MAC_N_LMNA

    Plasmid

    #111688

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    LMNA (LMNA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEF-DEST51-GOLPH3

    Plasmid

    #21690

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Invitrogen gateway destination vector

    Tags

    6xHIS and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pg-proPHD2-PHD2-EGFP

    Plasmid

    #87210

    Purpose

    PHD2-EGFP expressed under the control of the PHD2 promoter

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    PHD2 (EGLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    H313N

    Promoter

    PHD2

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV pCAG-mRuby3-WPRE

    Plasmid

    #107744

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Article

    Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge