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pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

Plasmid

#85749

Purpose

Expresses anti-CRISPR protein from Type I-E and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type I-E anti-CRISPR
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS443-pCSDest2-AcrIIC3Nme

Plasmid

#85713

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC3Nme

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP-CytERM-N-17

Plasmid

#56217

Purpose

Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CytERM

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Paxillin-22

Plasmid

#56234

Purpose

Localization: Focal Adhesions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Paxillin

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-MyosinIIC-N-18

Plasmid

#56232

Purpose

Localization: Cytoskeleton, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

MyosinIIC

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP-MannII-N-10

Plasmid

#56229

Purpose

Localization: Mannosidase/Golgi, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

MannII

Tags

mIFP

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEJS430-pCSDest2-AcrIIC1Boe

Plasmid

#85678

Purpose

Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

Type II-C anti-CRISPR AcrIIC1Boe

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-H2B-C-10

Plasmid

#55422

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

H2B (H2BC11 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Dectin1A-N-10

Plasmid

#55414

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Dectin1A-C-10

Plasmid

#55413

Purpose

Localization: Membrane, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Dectin1A (CLEC7A Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
BII-C3H2B

Plasmid

#133387

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP12-CAF1-C-10

Plasmid

#56246

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CAF1

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP24-CAF1-C-10

Plasmid

#56266

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CAF1

Tags

mIFP24

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi G418 Hu CD19

Plasmid

#183247

Purpose

Expression of human CD19 antigen in a Sleeping Beauty transposon vector

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

CD19 (CD19 Human)

Expression

Mammalian

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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px459-TDP-43 sgRNA1

Plasmid

#133762

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-TFE3

Plasmid

#38120

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

TFE3 (TFE3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-SiT-N-15

Plasmid

#55445

Purpose

Localization: Golgi TGN, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

SiT (ST6GAL1 Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only