We narrowed to 2,375 results for: pcas9

1. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTij_sg_mmu_Med1_N_terminal

   Plasmid

   #197870

   Purpose

   CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   CRSP210 (Med1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD1CB0001

   Plasmid

   #185625

   Purpose

   MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)

   Plasmid

   #159664

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)

   Plasmid

   #159663

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)

   Plasmid

   #159662

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

   Plasmid

   #159656

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBH-Cas9-Suntag-BB_BbsI

   Plasmid

   #164805

   Purpose

   REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330_gRNA_IG-DMR-2

    Plasmid

    #159932

    Purpose

    Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse IG-DMR
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CBh

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT46_GalL_cCDA1-BE3

    Plasmid

    #145039

    Purpose

    Expresses cCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    cCDA1-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT171_GalL_A3AΔ178-BE3

    Plasmid

    #145121

    Purpose

    Expressing base editor A3AΔ178-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ178-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-178AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT170_GalL_A3AΔ182-BE3

    Plasmid

    #145120

    Purpose

    Expressing base editor A3AΔ182-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ182-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-182AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT169_GalL_A3AΔ186-BE3

    Plasmid

    #145119

    Purpose

    Expressing base editor A3AΔ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ186-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT166_GalL_A3A-BE3

    Plasmid

    #145116

    Purpose

    Expressing base editor A3A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT139_GalL_nCDA1-VRERBE3

    Plasmid

    #145102

    Purpose

    Expressing base editor nCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VRERBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJT92_GalL_nCDA1Δ188-BE3

    Plasmid

    #145052

    Purpose

    Expresses nCDA1Δ188-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ188-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-188AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJT90_GalL_nCDA1Δ190-BE3

    Plasmid

    #145050

    Purpose

    Expresses nCDA1Δ190-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ190-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-190AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJT85_GalL_nCDA1Δ193-BE3

    Plasmid

    #145048

    Purpose

    Expresses nCDA1Δ193-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ193-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-193AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT84_GalL_nCDA1Δ194-BE3

    Plasmid

    #145047

    Purpose

    Expresses nCDA1Δ194-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ194-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-194AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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