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pCI 9.4

Plasmid

#74230

Purpose

Luminescent coincidence reporter with FLAG tags, FLuc-P2A-NLuc

Depositor

James Inglese

Article

Schuck et al Curr Protoc Neurosci. 2017 Apr 10;79:5.3

Insert

3xFlag-FLuc-P2A-3xFlag-NLuc

Expression

Mammalian

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-LEXY (pDN157)

Plasmid

#72658

Purpose

Constitutively nuclear LEXY variant for blocking endogenous, CRM-1-dependent nuclear export with blue light

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-LEXY

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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mV-NcWC1-LOV_368

Plasmid

#58691

Purpose

Expresses mVenus-fusion protein of NcWC1-LOV domain (Neurospora crassa White Collar-1)

Depositor

Harald Janovjak

Article

Grusch et al EMBO J. 2014 Jul 1. pii: e201387695.

Insert

mV-NcWC1-LOV

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pSLHDR_R4

Plasmid

#124211

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR04

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSLHDR_R1

Plasmid

#124209

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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MetNI (E166Q in MetN)

Plasmid

#78320

Purpose

Methionine ABC Transporter

Depositor

Douglas Rees

Article

Johnson et al Protein Sci. 2012 Jan;21(1):84-96. doi:

Inserts

MetI
MetN

Tags

10x Histidine Tag and 6x Histidine Tag

Expression

Bacterial

Mutation

E166Q

Promoter

T7 Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJG01

Plasmid

#213505

Purpose

expresses TdTomato in Capsaspora owczarzaki

Depositor

Joseph Gerdt

Insert

TdTomato

Promoter

EF1

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-Mito4x-GCaMP6f

Plasmid

#127870

Purpose

Fluorescent reporter for mitochondrial matrix Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

4 repeats of COX8 signal peptide plus GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Article

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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N-Cadherin-EGFP

Plasmid

#18870

Depositor

Valeri Vasioukhin

Article

Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

Insert

N-Cadherin (Cdh2 Mouse)

Tags

EGFP, Kanamycin, and N-Cadherin

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-NET-myc

Plasmid

#107458

Purpose

Mammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Norepinephrine Transporter (NET) (SLC6A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAct-Cas9

Plasmid

#62209

Purpose

Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

act5C

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pQC-CherryOFF-GFP

Plasmid

#129101

Purpose

Reporter vector

Depositor

Fangliang Zhang

Article

Birnbaum et al Cell Chem Biol. 2018 Aug 16;25(8):1038-1

Insert

CherryOFF

Use

Retroviral

Expression

Mammalian

Mutation

G-> A

Promoter

CMV

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-OMM-GCaMP6f

Plasmid

#127874

Purpose

Fluorescent reporter for mitochondrial outer membrane Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ZipGFP-Casp3

Plasmid

#81241

Purpose

A ZipGFP-based Caspase-3 protease reporter T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

ZipGFP with caspase cleavage sequence T2A mCherry

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-AsLOV2-NES27 (pDN158)

Plasmid

#72659

Purpose

Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-AsLOV2-NES27

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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