We narrowed to 3,492 results for: aaas

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Halo-TCAB1 KI sgRNA

Plasmid

#207611

Purpose

sgRNA for HaloTag insertion into the endogenous TCAB1 locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

CCAAAGTCTTCATACTGCAG

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ5135: pCAG-BFP-GuideArray

Plasmid

#210606

Purpose

Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

Depositor

Stanley Qi

Article

Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

Insert

pCAG-BFP-GuideArray

Use

CRISPR

Promoter

CAG

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PARP1

Plasmid

#183312

Purpose

All-in-One CRISPRko system with a guide RNA that targets PARP1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PARP1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shLuc

Plasmid

#83092

Purpose

Lentiviral shRNA vector for inducible knockdown of luciferase (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLuc

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only
hU6 promoter PspCas13b gRNA dual luciferase reporter 3’UTR

Plasmid

#119859

Purpose

gRNA plasmid with hU6 promoter PspCas13b gRNA dual luciferase reporter

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

gRNA dual luciferase reporter

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M4NS_AmpR

Plasmid

#119156

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region

Use

Crispr

Promoter

J23119

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

Plasmid

#172225

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-H1-SGIPZ_NF1 sh1miR

Plasmid

#119206

Purpose

knockdown of NF1

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

sh1miR targeting NF1 (NF1 Mouse, Human, Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 EB1 shRNA #3

Plasmid

#37927

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 shRNA #3 (MAPRE1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

human U6

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FLT3

Plasmid

#183293

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pARNTL.1.0-gDNA

Plasmid

#112480

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL

Depositor

Kevin White

Insert

ARNTL (BMAL1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-L1221

Plasmid

#132933

Purpose

Lentivector for luciferase (control) knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

luciferase shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-SMYD2_1

Plasmid

#36349

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SMYD2 (SMYD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_GART

Plasmid

#183296

Purpose

All-in-One CRISPRko system with a guide RNA that targets GART gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

GART

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPU-Empty-miR30-L1221

Plasmid

#132940

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Promoter

SFFV

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pPU-huTRIMmiR30-L1221

Plasmid

#132937

Purpose

All-in-one huTRIM5 rescue-control shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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double_sgRNA targeting e3 and e7

Plasmid

#190689

Purpose

sgRNAs targeting enhancer 3 and 7 of MYC separately

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNAs targeting enhancer 3 and 7 of MYC separately

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSR9.1347

Plasmid

#39540

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pSuper.Retro.Puro-WDR5 shRNA 4

Plasmid

#59977

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only