We narrowed to 29,570 results for: des.2

1. LB1461 (Bcl-2 promoter with MHS1234, NFkB mut)

   Plasmid

   #15385

   Depositor

   Linda Boxer

   Article

   Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

   Insert

   B-cell lymphoma/leukemia-2 (BCL2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Bacterial and Mammalian

   Mutation

   Bcl-2 full promoter with IgH full enhancer MHS123…

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

   Plasmid

   #227619

   Purpose

   Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   deleted aa 2-83

   Promoter

   CMV, T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

   Plasmid

   #197425

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MMW2993 - human expression plasmid for SpCas9 Cluster 2

   Plasmid

   #101185

   Purpose

   Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
   

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   G582A/V583A/E584A/D585A/N588A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV14-3X-Flag-SARS-CoV-2 S

   Plasmid

   #145780

   Purpose

   Over expression of SARS-CoV-2 S (spike) in mammalian cell line

   Depositor

   Zhaohui Qian

   Article

   Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

   Insert

   SARS-CoV-2 S (S SARS CoV 2)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Codon optimized and C-terminal 19 amino acids del…

   Promoter

   CMV

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

   Plasmid

   #209198

   Purpose

   Mutagenesis of Th

   Depositor

   Larry Zweifel

   Article

   Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

   Insert

   Th (Th Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Promoter

   CMV

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FH1-CLGT#2-mCherry-_PGK-H2B-CFP

   Plasmid

   #164106

   Purpose

   Fluorescent reporter for genetic tracing of classical Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   CLGT#2-mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

   Plasmid

   #224514

   Purpose

   A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xNLS-(G2SG2)2-Cre
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-U6-PALMDgRNA1+2-cTnT-Cre -mi122TS

   Plasmid

   #216830

   Purpose

   cardiomyocytes-specific plasmid, expressing gRNAs targeting Palmd exon7, containing a mi122TS sequence to decrease off-target effects in the liver.

   Depositor

   Yuxuan Guo

   Article

   Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

   Insert

   PALMDgRNA
   

   Use

   AAV

   Available Since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBiFC-flag-hCaspase 2 FL (C303S)-VN173

    Plasmid

    #49258

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLV-mScarlet-SARS-CoV-2-SL1 alone

    Plasmid

    #191485

    Purpose

    Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

    Plasmid

    #112842

    Purpose

    transient/stable overexpression of the central domain of Catulin.

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Catulin-alpha-1 (Ctnnal1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C1 domain of Catulin

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL-mouse cGAS #2-gRNA-Cas9-Puro

    Plasmid

    #186891

    Purpose

    gRNA targeting mouse cGAS (with Cas9 insert)

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Cas9 (Cgas Mouse)
    

    Use

    Lentiviral

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid

    #141184

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Has Service

    Cloning Grade DNA

    Insert

    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    

    Tags

    Signal peptide from influenza HA and Superfolder …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid

    #141183

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid

    #159390

    Purpose

    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

    Depositor

    Tarun Kapoor

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

    Plasmid

    #176060

    Purpose

    Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K164A and H165A

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 SARS-CoV-2 S D614G-trunc

    Plasmid

    #158077

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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