We narrowed to 2,321 results for: pcas9

1. pJT131_GalL_cCDA1-NGBE3

   Plasmid

   #145095

   Purpose

   Expressing base editor cCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-NGBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL1RN gRNA1

   Plasmid

   #113130

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for gRNA expression, RSV for GFP expression

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SIN-CMV-Ca9-WPRE

   Plasmid

   #87874

   Purpose

   Transfer plasmid for the production of lentiviral vectors

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   nls

   Expression

   Mutation

   Human codon-optimized

   Promoter

   CMV

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-sgRNA_Dicer_1

   Plasmid

   #68807

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

   Plasmid

   #234724

   Purpose

   All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

   Depositor

   Kirill Martemyanov

   Article

   Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   T2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ1023

   Plasmid

   #228763

   Purpose

   Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-TP53

   Plasmid

   #227318

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-MAPRE1

   Plasmid

   #207793

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-PITCh-MAP4

   Plasmid

   #227295

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of MAP4 (MAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-EZR

    Plasmid

    #227293

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of EZR (EZR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-PITCh-CEP192

    Plasmid

    #227288

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CEP192 (CEP192 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-CEP135

    Plasmid

    #227286

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-ARL13B

    Plasmid

    #227276

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of ARL13B (ARL13B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

    Plasmid

    #131683

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    EF1a and mU6

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCPH2A.X_K5Q-GS3

    Plasmid

    #204746

    Purpose

    Expression of Cas9 and human H2AX with the K5Q mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K5Q (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K5Q mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPH2A.X_K5R-GS3

    Plasmid

    #204747

    Purpose

    Expression of Cas9 and human H2AX with the K5R mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K5R (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K5R mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCPH2A.X_K134L-GS3

    Plasmid

    #204750

    Purpose

    Expression of Cas9 and human H2AX with the K134L mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134L (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134L mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCPH2A.X_K134M-GS3

    Plasmid

    #204751

    Purpose

    Expression of Cas9 and human H2AX with the K134M mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134M (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134M mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCPH2A.X_K134A-GS3

    Plasmid

    #204752

    Purpose

    Expression of Cas9 and human H2AX with the K134A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX K134A (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the K134A mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGMC00033 (aka pMC0224)

    Plasmid

    #210750

    Purpose

    Guide RNA targeting SERPINB3

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    SERBPINB3 guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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