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pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

Plasmid

#242651

Purpose

CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-ABE8.8m-SpCas9-P2A-EGFP

Use

CRISPR

Expression

Mammalian

Mutation

ABE8.8 mutations in TadA

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3c

Plasmid

#59422

Purpose

Expresses lncRNA MEG3 isoform c in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3c (MEG3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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AXBEv2

Plasmid

#204606

Purpose

Mammalian expression of evolved adenine transversion base editor AXBEv2

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-SQSTM1

Plasmid

#223715

Purpose

Expresses Flag-tagged SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CMV RasV12 Neo (w108-1)

Plasmid

#22259

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V mutant

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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LZ10 PBREBAC-H2BHalo

Plasmid

#91564

Purpose

piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.

Depositor

James Zhe Liu

Article

Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1

Insert

histone H2B-HaloTag

Tags

HaloTag

Expression

Mammalian

Promoter

EF1

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-LaminB1-10

Plasmid

#57141

Purpose

Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

LaminB1 (LMNB1 Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET303_sumocodo_GFP_codoopti

Plasmid

#210393

Purpose

Express SUMO-GFP in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

GFP

Tags

SUMO-tag

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (Zeocin)

Species

Escherichia coli

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only