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BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

Plasmid

#101178

Purpose

Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Publication

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

N692A/M694A/Q695A/H698A

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV4-ApoE2

Plasmid

#87085

Purpose

Expresses ApoE2 in Mammalian cells

Depositor

Bradley Hyman

Publication

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E2 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHaloTag-EGFP

Plasmid

#86629

Purpose

Expresses HaloTag-eGFP tandem (cytosolic)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCFD6

Plasmid

#73915

Purpose

tissue-specific multiplex gRNA plasmid

Depositor

Simon Bullock

Publication

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

:tRNA:gRNA:tRNA:gRNA:tRNA

Use

CRISPR

Expression

Insect

Promoter

UAS-DSCP

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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pZW6 (CK2alpha)

Plasmid

#27086

Depositor

David Litchfield

Publication

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pDS-RED-C1-Beclin1

Plasmid

#24405

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

DsRed

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMXs-gw

Plasmid

#18656

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Gateway cassette A

Use

Retroviral

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pBiFC-bJunVN173

Plasmid

#22012

Depositor

Chang-Deng Hu

Publication

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bJunVN173 (Jun Rat, Aequorea Victoria)

Tags

FLAG

Expression

Mammalian

Mutation

c-Jun(257-338)-Venus(1-172)

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pCDNA3 Flag MKK7B2Jnk1a1

Plasmid

#19726

Depositor

Roger Davis

Publication

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk1a1 (Map2k7, MAPK8 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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HA-VHL wt-pBabe-puro

Plasmid

#19234

Depositor

William Kaelin

Publication

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

wt

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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nuclear EKAR (Cerulean-Venus)

Plasmid

#18681

Depositor

Karel Svoboda

Publication

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Tags

monomeric Cerulean and monomeric Venus

Expression

Mammalian

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pRK5-Myc-Parkin

Plasmid

#17612

Depositor

Ted Dawson

Publication

Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

Insert

Parkin (PRKN Human)

Tags

Myc

Expression

Mammalian

Available since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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PGKdtabpA

Plasmid

#13440

Depositor

Philippe Soriano

Publication

Soriano et al Development. 1997 Jul . 124(14):2691-700

Insert

PGKdtabpA

Expression

Mammalian

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pcDNA3-EGFP-Cdc42-wt

Plasmid

#12975

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pIS0

Plasmid

#12178

Purpose

3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

Depositor

David Bartel

Publication

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (AAV5)

Viral prep

#100845-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 (#100845). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6s.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Publication

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Promoter

Syn

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only
L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Publication

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Publication

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pK7WGF2::hCas9

Plasmid

#46965

Purpose

Expresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

hCas9

Use

CRISPR; Plant expression

Tags

eGFP

Promoter

35S

Available since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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