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pCAG-Izumo1-1D4

Plasmid

#183544

Purpose

Expression vector of mouse izumo sperm-egg fusion 1 (Izumo1) tagged with 1D4 at C-terminus.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

izumo sperm-egg fusion 1 (Izumo1 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4404

Plasmid

#115465

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4404 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX SgK269 WT

Plasmid

#68028

Purpose

expression of Sgk269 in mammalian cells

Depositor

Roger Daly

Article

Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

Insert

PEAK1 (PEAK1 Human)

Use

Retroviral

Tags

FLAG/myc, HA, and IRES-DsRed

Expression

Mammalian

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cn-VA-NHEJ1-WT

Plasmid

#141339

Purpose

Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1 (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-SNAPf-CCR5TC

Plasmid

#135536

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

SNAPf

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Control-cx43.4-48H-pGTag

Plasmid

#117818

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-xBE4Gam-P2A-Puro

Plasmid

#112676

Purpose

Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE4Gam

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.V2

Plasmid

#127848

Purpose

non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B4

Plasmid

#127849

Purpose

non-standard AAV2 rep-AAV-PHP.B4 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.B4 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-TagBFP

Plasmid

#117777

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-Luc

Plasmid

#122034

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

Luciferase

Use

AAV

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-eGFP

Plasmid

#117779

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shSNAI1

Plasmid

#115467

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shSNAI1 (SNAI1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-MBNL2

Plasmid

#115444

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL2 (MBNL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-B-actin

Plasmid

#117814

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Olig1 [N149/25.1R]

Plasmid

#114540

Purpose

Mammalian Expression Plasmid of anti-Olig1 (Mouse). Derived from hybridoma N149/25.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Olig1 (Mus musculus) recombinant mouse monoclonal antibody (Olig1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-FLAG-TAOK2

Plasmid

#197862

Purpose

Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

Depositor

Jonathan Morris

Article

Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

Insert

TAOK2 (TAOK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-mRFP

Plasmid

#117772

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-B-actin

Plasmid

#117808

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-5 [N195A/16R]

Plasmid

#114481

Purpose

Mammalian Expression Plasmid of anti-QKI-5 (Human). Derived from hybridoma N195A/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-QKI-5 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-MMACHC [N230/21R]

Plasmid

#114555

Purpose

Mammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-cmlc2-mRFP

Plasmid

#117781

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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dCas9-NLS-gs10-cLYN268

Plasmid

#188259

Purpose

Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

cLYN268 (LYN Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-eGFP

Plasmid

#117794

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-xBE3-P2A-Puro

Plasmid

#110870

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE3

Use

Lentiviral

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR1A-HA-Jag1

Plasmid

#46052

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Use

Entry vector

Tags

HA tag

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pPRISM-gal4-VP16-cmlc2-TagBFP

Plasmid

#117780

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge