We narrowed to 4,319 results for: 1-Oct
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Plasmid#209644PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100N
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro
Plasmid#209643PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100C
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 CortW22A mCherry
Plasmid#187268PurposeLenticiral expression of mCherry-Cortactin W22ADepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmcherryExpressionMutationW22APromoter5' LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#173211PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
UseCRISPR; Prime editing (pe3)TagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA
Plasmid#173212PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-bPAC(R278A)-Myc-eYFP
Plasmid#165488PurposeXenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activityDepositorInsertbPAC(R278A)-Myc-eYFP
UseCdna expression, xenopus oocyteTagsEYFP and MycExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-TPD53
Plasmid#172461PurposeExpression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFPDepositorInsertTPD53 (TPD52L1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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NH2end ELL2
Plasmid#127252PurposeExpresses the N terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (1-287) (ELL2 Human)
UseOtherTagsHis6XExpressionMammalianMutationcontains only amino acids 1-289PromoterpEF1aAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-I894A(pL84)
Plasmid#124404PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.DepositorInsertGal4-TET1-878-911(I894A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationI894APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-T898A(pL84)
Plasmid#124403PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.DepositorInsertGal4-TET1-878-911(T898A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationT898APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L900A(pL84)
Plasmid#124402PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.DepositorInsertGal4-TET1-878-911(L900A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL900APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L897A(pL84)
Plasmid#124401PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.DepositorInsertGal4-TET1-878-911(L897A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL897APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L900A(pL91)
Plasmid#124395PurposeE.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L900A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationL900APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898E(pL91)
Plasmid#124394PurposeE.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898E (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationT898EPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-T898A(pL91)
Plasmid#124393PurposeE.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-T898A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationT898APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L897A(pL91)
Plasmid#124392PurposeE.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L897A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationL897APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-I894A(pL91)
Plasmid#124391PurposeE.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-I894A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationI894APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson
Plasmid#117663PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT2)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HeFm1SpCas9
Plasmid#92110PurposeExpression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationR661A, Q695A, K848A, Q926A, K1003A, R1060APromoterCbhAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only