We narrowed to 4,217 results for: Alk

1. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

   Plasmid

   #62581

   Purpose

   Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   miR-21 binding site 2 mutant (TAAGCTA --> TAGA…

   Promoter

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pNK3071

   Plasmid

   #219755

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK497

   Plasmid

   #219754

   Purpose

   MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-kz-CD20-Puro

   Plasmid

   #209759

   Purpose

   Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY0209 ACTB Nicking guide +48

   Plasmid

   #179109

   Purpose

   ACTB N-term Nicking guide 1 +48 guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term Nicking guide 1 +48 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDY0216 PASTEv1 PE2-P2A-Bxb1

   Plasmid

   #179104

   Purpose

   PASTEv1 PE2-P2A-Bxb1

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-PE2-P2A-Bxb1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDY0663 ACTB atgRNA with v2 scaffold

   Plasmid

   #179108

   Purpose

   ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-DiCas7-11-His

   Plasmid

   #234482

   Purpose

   Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR-XL-rAkap9

   Plasmid

   #196867

   Purpose

   Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akap9 (Akap9 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter and T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223-MEKDD

    Plasmid

    #31202

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    MAPKK1 (MAP2K1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    S218D S222D

    Promoter

    Available since

    June 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pBactin-EB3-mNeonGreen

    Plasmid

    #196848

    Purpose

    labelling of microtubule plus ends

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    EB3-NeonGreen (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LCV2_mCherry_AAVS1_sgRNA_2

    Plasmid

    #155105

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. hSyn-GtACR2-ChRmine-tandem-eYFP

    Plasmid

    #192580

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    EYFP- soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-V3-CD20-Puro

    Plasmid

    #209757

    Purpose

    Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pINDUCER22-MKK6E-Flag

    Plasmid

    #99259

    Purpose

    Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

    Depositor

    Alejandro Adam, Peter Vincent

    Article

    Adam et al PLoS One. 2016 Sep 7;11(9):e0161975. doi

    Insert

    MKK6E (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S207E T211E

    Promoter

    TRE2

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-hRedOpsin-fCX3CL1

    Plasmid

    #164421

    Purpose

    AAV plasmid expressing full-length CX3CL1 (fractalkine) in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    Cx3cl1 (Cx3cl1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human red opsin

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDY0207 ACTB atgRNA with v1 scaffold

    Plasmid

    #179107

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCKO_AAVS1_sgRNA_2

    Plasmid

    #155085

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY0310 TP901-1 Integrase

    Plasmid

    #179110

    Purpose

    TP901-1 Integrase expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    TP901-1 Integrase
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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