We narrowed to 29,570 results for: des.2

1. 3159_pETcon-SARS-CoV-2-RBD-L452R-T478K

   Plasmid

   #184407

   Purpose

   yeast surface display of the SARS-CoV-2 Delta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Delta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   L452R+T478K

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234370

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234372

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-24XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-mouse STING #2-gRNA-Cas9-Puro

   Plasmid

   #186893

   Purpose

   gRNA targeting mouse STING (with Cas9 insert)

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Cas9 (Sting1 Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-22b(+)_PelB-BN00N0-2-FLAG-His

   Plasmid

   #218045

   Purpose

   RESOLUTE SLC binder expression plasmid

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Insert

   BN00N0-2
   

   Tags

   1xFLAG 10xHis and PelB

   Expression

   Bacterial

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-22b(+)_PelB-BN00A4-2-FLAG-His

   Plasmid

   #218071

   Purpose

   RESOLUTE SLC binder expression plasmid

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Insert

   BN00A4-2
   

   Tags

   1xFLAG-10xHis and PelB

   Expression

   Bacterial

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-22b(+)_PelB-BN00FH-2-FLAG-His

   Plasmid

   #218037

   Purpose

   RESOLUTE SLC binder expression plasmid

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

   Insert

   BN00FH-2
   

   Tags

   1xFLAG 10xHis and PelB

   Expression

   Bacterial

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGHUC w-ERBIN (2-hybrid prey plasmid)

   Plasmid

   #128163

   Purpose

   UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

   Depositor

   Marcus Noyes

   Article

   Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

   Insert

   ERBIN PDZ domain (ERBIN Synthetic)
   

   Use

   Synthetic Biology

   Tags

   FLAG and Omega subunit of RNA polymerase

   Expression

   Bacterial

   Promoter

   UV5

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

   Plasmid

   #173030

   Purpose

   To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   SARS-CoV-2 Nucleocapsid phosphoprotein
   

   Use

   Lentiviral

   Tags

   3xHA and IRES-mcherry

   Expression

   Mammalian

   Promoter

   Gag (truncated)

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR207 SARS-CoV-2 E 27nt-del

    Plasmid

    #153954

    Purpose

    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pDONR207 SARS-CoV-2 E 27nt-del_nostop

    Plasmid

    #153955

    Purpose

    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 23, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169193

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    14His-SUMO

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169192

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Unspecified

    Tags

    6His-TEV

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. SARS CoV-2 HexaPro spike (bio-His)

    Plasmid

    #166856

    Purpose

    Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.

    Depositor

    Evelien Bunnik

    Article

    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

    Insert

    SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
    

    Tags

    8xHis and Biotinylation site

    Expression

    Mammalian

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 SARS-CoV-2 S 24nt-del

    Plasmid

    #153952

    Purpose

    Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 23, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pDONR223 SARS-CoV-2 S 24nt-del_nostop

    Plasmid

    #153953

    Purpose

    Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

    Plasmid

    #153956

    Purpose

    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pDONR223 SARS-CoV-2 ORF7B C-trunc

    Plasmid

    #153957

    Purpose

    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Anti-CDY1/2/L1/L2 [N143/36R]

    Plasmid

    #206534

    Purpose

    Mammalian Expression Plasmid of anti-CDY1/2/L1/L2 (Human). Derived from hybridoma N143/36.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-CDY1/2/L1/L2 (Homo sapiens) recombinant Mouse monoclonal antibody (CDY1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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