We narrowed to 5,083 results for: mcs

1. 3542 pMIG Bcl-XL mut1

   Plasmid

   #8791

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bcl-XL (BCL2L1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   F131V, D133A

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET21-pelB-LaG-8

   Plasmid

   #172761

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-8
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaG-5

   Plasmid

   #172760

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-5
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRC/CMV-TYK2V362F-VSV

   Plasmid

   #139345

   Purpose

   Expression of TYK2 V362F protein (rs2304256)

   Depositor

   Sandra Pellegrini

   Article

   Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   V362F

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL-SV40-Rac1 5'-UTR

   Plasmid

   #115354

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   Rac1 5'-UTR (RAC1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL1-TTlight-4xDQNAT

   Plasmid

   #128394

   Purpose

   In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Tags

   4xDQNAT glycosylation tag and 6xHis tag

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   T7

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFL-SV40-GNB2 5'-UTR

   Plasmid

   #115355

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   GNB2 5'-UTR (GNB2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaG-2

   Plasmid

   #172739

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-uTEV1-MAVS-mTA

   Plasmid

   #170999

   Purpose

   HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[R537A]
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[R537A]

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTrc99S-ssDsbA-PorA-4xDQNAT

    Plasmid

    #128400

    Purpose

    Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    N. meningitidis PorA
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bov.hsc70.RSET.a1-554.wt(NarI)

    Plasmid

    #12533

    Depositor

    David McKay

    Article

    Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

    Insert

    Bovine 70 kDa heat shock cognate protein fragment amino acids 1-554
    

    Expression

    Bacterial

    Mutation

    deleted carboxy-terminal residues 555-650 of bovi…

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSP64-hERG1a F656A

    Plasmid

    #53054

    Purpose

    Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Mutation

    changed Phenylalanine 656 to Alanine

    Promoter

    SP6

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSP64-hERG1a Y652A

    Plasmid

    #53053

    Purpose

    Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Mutation

    changed Tyrosine 652 to Alanine

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL1-aadA

    Plasmid

    #117050

    Purpose

    In vitro expression of aadA (streptomycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    aadA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIPZ-2xFLAG-2xSTREP-E2F3A

    Plasmid

    #236435

    Purpose

    doxycycline inducible expression of E2F3A in mammalian cells

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3A (E2f3 Mouse)
    

    Tags

    FLAG-FLAG-STREP-STREP

    Expression

    Mammalian

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-Cherry-PCNA-P2A-Venus-E2F3

    Plasmid

    #236448

    Purpose

    insect expression of E2F3 for protein purification

    Depositor

    Michele Pagano

    Article

    Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

    Insert

    E2F3 (E2f3 Mouse)
    

    Expression

    Insect

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. EW375 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-GFP-deimlink-NZF

    Plasmid

    #236177

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and GFP-NZF dummy transcription factor

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    EGFP-NZF
    H2B (H2BC11 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and four ZNF35/IKZF1 sites

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pk335-CARGO-6mer-73kb-DSF

    Plasmid

    #227499

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 73kb Downstream Fgf5
    

    Use

    CRISPR

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pk335-CARGO-6mer-26kb-DSF

    Plasmid

    #227482

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 26kb Downstream Fgf5
    

    Use

    CRISPR

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pk335-CARGO-6mer-30kb-DSF

    Plasmid

    #227483

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 30kb Downstream Fgf5
    

    Use

    CRISPR

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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