We narrowed to 14,304 results for: TIM;

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pFH77

Plasmid

#125816

Purpose

Level0 enCas9-PolI3M-TBD, wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

enCas9-PolI3M-TBD, wheat codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH91

Plasmid

#125819

Purpose

Level0 nScCas9 (nickase), wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nScCas9 (nickase), wheat codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ292

Plasmid

#102686

Purpose

S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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KFP08

Plasmid

#119798

Purpose

SigF2 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1784

Expression

Bacterial

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRec-GalS

Plasmid

#104573

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-GalS chimera constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI-galS

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRec-TreR

Plasmid

#104574

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-TreR chimera constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI-treR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal DsRedEx2

Plasmid

#104024

Purpose

Plasmid encoding 2ndVal DsRed-Express2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal DsRed-Express2

Use

Low copy

Expression

Bacterial

Mutation

DsRed-Express2 with valine inserted in 2nd positi…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDA199

Plasmid

#74275

Purpose

pSTL1-dPSTRy(4,3)

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

pSTL1-dPSTRy(4,3)

Expression

Bacterial and Yeast

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b-eIF4E-S200C

Plasmid

#37229

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4E

Expression

Bacterial

Mutation

S200C

Promoter

T7

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
pTYB2-eIF4B-A130C

Plasmid

#37227

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Expression

Bacterial

Mutation

A130C

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pUC19-DAD4

Plasmid

#37224

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

DAD4 (DAD4 Budding Yeast)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pAJM.712

Plasmid

#108513

Purpose

PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCymRC-YFP

Expression

Bacterial

Available Since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.714

Plasmid

#108515

Purpose

PVanCC-YFP Reporter. Van sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PVanCC-YFP

Expression

Bacterial

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_BE4max

Plasmid

#112093

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax_P2A_GFP

Plasmid

#112101

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pWRS_1001

Plasmid

#192205

Purpose

lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

Depositor

William Sellers

Article

Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and RNAi

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_501

Plasmid

#96922

Purpose

for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMOS007: PercevalHR ATP/ADP sensor (cytosolic)

Plasmid

#163061

Purpose

PercevalHR ATP/ADP sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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