We narrowed to 8,939 results for: pcDNA3

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pcDNA3.1(+)- e18 LOF

Plasmid

#131729

Purpose

Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Drp1 V2 S579A

Plasmid

#46342

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Expression

Mammalian

Mutation

S579A

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

Plasmid

#67299

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMagFast2(3x)-iRFP (Tiam1 Mouse, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pY129 (pcDNA3.1-U6-huMb3Cpf1)

Plasmid

#92305

Purpose

Expression of humanized Mb3Cpf1 and crRNA

Depositor

Feng Zhang

Insert

huMb3Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR-Kras

Plasmid

#123357

Purpose

FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR-Kras

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc S36A

Plasmid

#10973

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Mutation

S36A

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-Mito-CaNARi

Plasmid

#64851

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean, N-terminal 30 amino acids from DAKAP1, …

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP

Plasmid

#214367

Purpose

Expression of the Blue Fluorescent Protein

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K5-9R

Plasmid

#63562

Purpose

Expression of human H2A with K5R and K9R tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysine 5 and Lysine 9 are now Arginine

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CnA-H151A

Plasmid

#191956

Purpose

Expression of phosphatase dead mutant of calcineurin A (CnA-H151A).

Depositor

Luca Scorrano

Insert

Calcineurin subunit alpha H151A

Expression

Mammalian

Mutation

H151A

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 ΔC (1-381)

Plasmid

#62973

Purpose

Expresses HA-tagged rat SOX9 residues 1-383

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA

Expression

Mammalian

Mutation

codes for residues 1-381

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-Rheb(5A)

Plasmid

#192439

Purpose

Encodes mCherry-tagged Rheb lacking residues 38-42.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb amino acids 38-42 deleted.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pY127 (pcDNA3.1-U6-huTsCpf1)

Plasmid

#92303

Purpose

Expression of humanized TsCpf1 and crRNA

Depositor

Feng Zhang

Insert

huTsCpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PA-Cre-Y324F

Plasmid

#122961

Purpose

Catalytically inactive mutant of PA-Cre

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_PA-Cre-Y324F

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 EYFP H148Q

Plasmid

#25871

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with Histidine 148 to …

Available Since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only
Cav2.2 BBS pcDNA3

Plasmid

#206057

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-AP1S3

Plasmid

#58292

Purpose

mammalian expression of AP1S3

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only