We narrowed to 12,012 results for: pcdna

1. pcDNA3.1-Cry2FL-VP64

   Plasmid

   #60554

   Purpose

   Expresses Cry2FL-VP64 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   Cry2FL-VP64
   

   Use

   Tags

   FLAG, HA tag, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-Lin28A

   Plasmid

   #51371

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   Lin28A (LIN28A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dead-Laconic/pcDNA3.1(-)

   Plasmid

   #118627

   Purpose

   To express in mammalian cells a lactate FRET sensor negative control.

   Depositor

   Luis Felipe Barros

   Article

   Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

   Insert

   Dead-Laconic
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Change Histidine 151 to Aspartic Acid of Escheric…

   Promoter

   CMV Promoter

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) EGFP-RalA(WT)

   Plasmid

   #224274

   Purpose

   Cell transfection and expression of RalA

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Casp2-Flag

   Plasmid

   #11811

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 (CASP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT/TO_TOMM20_HaloTag7

   Plasmid

   #169330

   Purpose

   Expression of TOMM20-HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag7 (TOMM20 Human)
   

   Use

   Tags

   HaloTag7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-YFP-PKD1

   Plasmid

   #31527

   Depositor

   Alexandra Newton

   Article

   Kunkel et al J Biol Chem. 2009 Sep 4. 284(36):24653-6

   Insert

   YFP-HA-Protein Kinase D (PRKD1 Human)
   

   Use

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1/hChR2-EYFP

   Plasmid

   #20939

   Purpose

   Mammalian expression of humanized ChR2 fused to EYFP

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-PPO-mScarlet

   Plasmid

   #139503

   Purpose

   Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Insert

   parapinopsin
   

   Use

   Tags

   mScarlet following Gly-Gly-Ser linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid

    #52421

    Purpose

    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

    Depositor

    Bertrand Collet

    Insert

    Fusion NLS-EGFP-P2A-Cherry-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 3xF-dyskerin

    Plasmid

    #126870

    Purpose

    Expresses 3xFlag dsykerin

    Depositor

    Kathleen Collins

    Article

    Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

    Insert

    Dyskerin
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sACE2(WT)-Fc(IgA1)

    Plasmid

    #145154

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgA1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.3-3XFLAG-AGO2

    Plasmid

    #136687

    Purpose

    Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

    Depositor

    David Bartel

    Article

    McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

    Insert

    argonaute RISC Catalytic Component 2 (AGO2 Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. hKCNQ3(WT).ires.mCherry-pcDNA3

    Plasmid

    #204362

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 HA-humanTRAF3 (1098)

    Plasmid

    #66927

    Purpose

    Mammalian Expression of TRAF3

    Depositor

    Michael Karin

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

    Plasmid

    #169328

    Purpose

    Expression of HaloTag9 located at the microtubules of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag9 (CEP41 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 proHB-EGF NC

    Plasmid

    #11605

    Depositor

    Rosalyn Adam

    Article

    Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

    Insert

    HB-EGF (HBEGF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

    Promoter

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-ExRai-AktAR

    Plasmid

    #118462

    Purpose

    cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 CLN3-GFP

    Plasmid

    #78110

    Purpose

    Mammalian expression of human CLN3 with C terminal GFP fusion

    Depositor

    Thomas Braulke

    Insert

    CLN3 (CLN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

20. FLAG-3C-sGFP1-10_pcDNA3.1(-)

    Plasmid

    #182931

    Purpose

    For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

    Depositor

    William Weis

    Article

    Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

    Type

    Empty backbone

    Use

    Tags

    3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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