We narrowed to 4,217 results for: Alk

1. pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

   Plasmid

   #222289

   Purpose

   Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   KIF1C (KIF1C Human)
   

   Use

   Tags

   6His and GST

   Expression

   Bacterial

   Mutation

   stalk region only amino acids 642 to 922

   Promoter

   T7

   Available since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-RfxCas13d-hei

   Plasmid

   #234485

   Purpose

   Plasmid to carry out IVT of RfxCas13d with myc tag and two oNLS (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   myc and oNLS

   Expression

   Mutation

   Promoter

   Available since

   April 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK845

   Plasmid

   #219746

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal luciferase
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   I3S, N4T, F11L, I63T, T99P, T192S, A199P

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-V1-CD20-Puro

   Plasmid

   #209755

   Purpose

   Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-V2-CD20-Puro

   Plasmid

   #209756

   Purpose

   Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.

   Depositor

   Andrei Thomas-Tikhonenko

   Article

   Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

   Insert

   MS4A1 (MS4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

   Plasmid

   #196879

   Purpose

   Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-rox-inv[Talpha1-iCre-pA]-rox-mNeonGreen-hCentrin1

   Plasmid

   #196892

   Purpose

   Neuron-specific expression of human (h)Centrin fused to mNeonGreen. Used in combination with Talpha1-Dre-pA plasmids

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mNeonGreen-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1

   Plasmid

   #196891

   Purpose

   Neuron-specific expression of human (h)Centrin fused to 2xHA-tags

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   2xHA-hCentrin1 (CETN1 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBactin-Nedd1-mOrange2-rCM1

   Plasmid

   #196861

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBactin-Nedd1-mOrange2-F75A

    Plasmid

    #196862

    Purpose

    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-F75A (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F75A in the CM1 domain

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBactin-Akna-mOrange2

    Plasmid

    #196865

    Purpose

    Expression of the centrosomal protein Akna fused to mOrange2

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Akna-mOrange2 (Akna Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI

    Plasmid

    #148949

    Purpose

    Mammalian Expression of HsGIGYF2_1-718-del280-310

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF2_1-718-del280-310 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

    Plasmid

    #148520

    Purpose

    Mammalian Expression of HseIF4G1-L640AV646A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-L640AV646A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

    Plasmid

    #148521

    Purpose

    Mammalian Expression of HseIF4G1-LIVVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-LIVVtoAAAA (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD

    Plasmid

    #148522

    Purpose

    Mammalian Expression of HseIF4G1-Y619AL624AL625A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-Y619AL624AL625A (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACE-Truncated-Kir3.2-StreptagII

    Plasmid

    #177263

    Purpose

    Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

    Insert

    G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
    

    Use

    Tags

    StreptagII

    Expression

    Insect

    Mutation

    Residues 49-378

    Promoter

    polyhedrin promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCV2_mClover3_PSMD1_sgRNA_1

    Plasmid

    #155102

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    PSMD1_sgRNA_1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LCV2_mClover3_AAVS1_sgRNA_4

    Plasmid

    #155101

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLCKO_AAVS1_sgRNA_3

    Plasmid

    #155086

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LCV2_mCherry_AAVS1_sgRNA_4

    Plasmid

    #155106

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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