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Plasmid#217172PurposeMammalian expression vector for expression of hAT-13_Ami transposase (CMV promoter)DepositorInserthAT-13_AMi transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 WT
Plasmid#212946PurposeVector used for expression of AtSLAC1 WT in mammalian cellDepositorInsertSlow anion channel-associated 1 (OZS1 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 6D
Plasmid#212947PurposeVector used for expression of AtSLAC1 6D in mammalian cellDepositorInsertSlow anion channel-associated 1 (OZS1 Mustard Weed)
UseTagsExpressionMammalianMutationT62D/S65D/S107D/S124D/S146D/S152DPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.3 (19-361)
Plasmid#177846PurposeBacterial Expression of SnRK2.3DepositorInsertSNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-Citrine-ADPG2
Plasmid#170725PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsCitrine tag (714bp)ExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-ADPG2
Plasmid#170723PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV_eA3A T31A-NG-nCas9
Plasmid#163539PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9
Plasmid#163550PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-mRIPK1-S321A
Plasmid#115339PurposeMammalian expression of Flag-mRIPK1-S321ADepositorInsertRIPK1 (Ripk1 Mouse)
UseTagsFlagExpressionMammalianMutationS321APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D
Plasmid#112986PurposeP element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genomeDepositorInsertmei-S332 (mei-S332 Fly)
UseP insertion vectorTagsGFPExpressionMutationT331DPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A
Plasmid#112988PurposeP element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genomeDepositorInsertmei-S332 (mei-S332 Fly)
UseP insertion vectorTagsGFPExpressionMutationT331APromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRA/PINCO
Plasmid#108938PurposeRetroviral expression of mRIN2 dRADepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationDeletion of RA domain (aa 751-836)PromoterLTRAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIN2 dRHVPS9/PINCO
Plasmid#108939PurposeRetroviral expression of mRIN2 dRHVPS9DepositorInsertRas and Rab interactor 2 (Rin2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationDeletion of RHVPS9 domain (aa 455-738)PromoterLTRAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
AF10_OM-LZ
Plasmid#111753PurposeBacterial expression for structure determinationDepositorInsertMLLT10 (MLLT10 Human)
UseTags6x His - TEVExpressionBacterialMutationContains the OM-LZ region of AF10 (residues 720-7…PromoterT7Available sinceJune 14, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf6(WT)-mCherry
Plasmid#79422PurposeExpresses C-terminally mCherry-tagged Arf6(WT) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.6 (11-362)
Plasmid#177847PurposeBacterial Expression of SnRK2.6DepositorInsertSNF1-related protein kinase 2.6 (OST1 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYEE
Plasmid#159750PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgNRF2-3
Plasmid#186838Purposeknock out NRF2 in mammalian cellsDepositorInsertNrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only