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Plasmid#154945PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-BiPOLES-mCerulean
Plasmid#154950PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS2333_Tier3(Lenti_inverse)-YPet_Puro
Plasmid#169664PurposeTier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)DepositorInsertPRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / RPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean
Plasmid#154951PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorHas ServiceAAV Retrograde, AAV5, and AAV9InsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA:NbVPE1a/b
Plasmid#223218PurposeExpresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoterDepositorInsertNbVPE1a/b
UseCRISPR and Synthetic BiologyTagsNoneExpressionPlantMutationPromoterArabidopsis U6 promoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChIEF-citrine
Plasmid#50974PurposeAAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITRDepositorInsertChIEF-citrine
UseAAVTagscitrineExpressionMutationChIEF gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChIEF-tdTomato
Plasmid#50977PurposeAAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITRDepositorInsertChIEF-tdTomato
UseAAVTagstdTomatoExpressionMutationChIEF gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChEF-citrine
Plasmid#50975PurposeAAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITRDepositorInsertChEF-citrine
UseAAVTagscitrineExpressionMutationChEF gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable sinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP2-His
Plasmid#67870Purposebacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tagDepositorInsertPRORP2 (PRORP2 Mustard Weed)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP3-His
Plasmid#67871Purposebacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tagDepositorInsertPRORP3 (PRORP3 Mustard Weed)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GtACR2-ChRmine-tandem-eYFP
Plasmid#192580PurposeAAV-vector for bidirectional optogenetic manipulation of neuronsDepositorInsertsomBiPOLES
UseAAVTagsEYFP- soma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-PdCO-EGFP-WPRE
Plasmid#198513PurposeExpresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-DIO-PdCO-mScarlet-WPRE
Plasmid#198511PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-PdCO-mScarlet-WPRE
Plasmid#198510PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAVTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-SIO-PdCO-mScarlet-WPRE
Plasmid#198509PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.DepositorInsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHES945
Plasmid#112643Purposeblue light inducible cryptochrome based optogenetic expression systemDepositorInsertCRY2PHR-GAL4DBD
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE
Plasmid#20294PurposeLentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to SerinePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE
Plasmid#20293PurposeLentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to AlaninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
Plasmid#20295PurposeLentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to ThreoninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-flex-oChIEF-citrine
Plasmid#50973Purposeflexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITRDepositorInsertChIEF-citrine
UseAAVTagscitrineExpressionMutationinverted and inserted between two lox sitesPromoterhuman synapsin promoterAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only