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We narrowed to 4,839 results for: pcr

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Showing: 201 - 220 of 4839 results

  7. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    53225-plasmid-map-sequence-id-82901

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  12. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

    Plasmid
    #211942
    Purpose
    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)
    Depositor
    Evgeny Kvon
    Publication
    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Expression
    Mammalian
    Promoter
    HSP68
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    211942-plasmid-map-sequence-id-426466

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  13. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    74293-plasmid-map-sequence-id-140421

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  14. pAc5 HA3-eDHFR-T2A-puro

    Plasmid
    #86395
    Purpose
    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection
    Depositor
    David Bentley
    Publication
    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA3 eDHFR T2A puro
    Use
    CRISPR
    Tags
    HA3 eDHFR T2A puro
    Mutation
    R12Y, G67S and Y100I mutations in E coli DHFR
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86395-plasmid-map-sequence-id-170910

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  15. pAc5 HA3-eDHFR-T2A-neo

    Plasmid
    #86396
    Purpose
    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection
    Depositor
    David Bentley
    Publication
    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HA3 eDHFR T2A neo
    Use
    CRISPR
    Tags
    HA3 eDHFR T2A neo
    Mutation
    R12Y, G67S and Y100I mutations in E coli DHFR
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    86396-plasmid-map-sequence-id-170909

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  20. pVITRO1-dV-IgE/κ

    Plasmid
    #61878
    Purpose
    Human immunoglobulin epsilon antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Publication
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin Epsilon constant region
    Human immunoglobulin kappa constant region
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61878-plasmid-map-sequence-id-101191

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Showing: 201 - 220 of 4839 results