We narrowed to 851 results for: streptococcus -(crispr) -(cas9)
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Plasmid#140569PurposeExpresses evoCas9 in mammalian cellsDepositorInsertCas9
MutationM495V Y515N K526E R661QAvailable SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialPromoterT7Available SinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc-y1sgRNA-Cas9
Plasmid#49331PurposeExpresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertsUseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL103-SpCas9-FLAG-3XNLS
Plasmid#182032PurposeExpression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNPDepositorInsertStreptococcus pyogenes cas9 (cas9 Synthetic)
UseCRISPRTags12X His tag, 3XNLS, FLAG, and MBPExpressionBacterialPromoterT7Available SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDicAID_nCas9-PmCDA_NptII_Della
Plasmid#91694PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDellaDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable SinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196084Purpose(Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseCRISPRExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26
Plasmid#149346PurposeMammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNADepositorInsertCas9
UseCRISPRTagsFlag tagExpressionMammalianPromoterCbcAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMK
Plasmid#62818PurposeBacterial constitutive S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSSBIO32_dCas9_RPA1786
Plasmid#240489PurposeCRISPRi-mediated Knockdown of RPA1786DepositorInsertCas9 (cas9 Streptococcus pyogenes)
UseCRISPRExpressionBacterialMutationD10A, H840APromoterPlacAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSSBIO32_dCas9_RPA0650
Plasmid#240487PurposeCRISPRi-mediated Knockdown of RPA0650DepositorInsertCas9 (cas9 Streptococcus pyogenes)
UseCRISPRExpressionBacterialMutationD10A, H840APromoterPlacAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSSBIO32_dCas9_RPA3717
Plasmid#240491PurposeCRISPRi-mediated Knockdown of RPA3717DepositorInsertCas9 (cas9 Streptococcus pyogenes)
UseCRISPRExpressionBacterialMutationD10A, H840APromoterPlacAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSSBIO32_dCas9_RPA3701
Plasmid#240490PurposeCRISPRi-mediated Knockdown of RPA3701DepositorInsertCas9 (cas9 Streptococcus pyogenes)
UseCRISPRExpressionBacterialMutationD10A, H840APromoterPlacAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSSBIO32_dCas9_RPA1580
Plasmid#240488PurposeCRISPRi-mediated Knockdown of RPA1580DepositorInsertCas9 (cas9 Streptococcus pyogenes)
UseCRISPRExpressionBacterialMutationD10A, H840APromoterPlacAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS9_10xHis_MBP_TEV_dCas9
Plasmid#244831PurposeBacterial expression of catalytically inactive Cas9 (dCas9) for protein purificationDepositorInsertdCas9 (WT)
UseCRISPRTags10xHis-MBPExpressionBacterialMutationD10A/H840APromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKMW124_dCas9
Plasmid#244821PurposeMammalian expression of catalytically inactive Cas9 (dCas9)DepositorInsertdCas9 (WT)
UseCRISPRTags3xFLAG-SV40 NLS and Nucleoplasmin NLSExpressionMammalianMutationD10A/H840APromoterCAG, U6Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-EGFP_cU6:6-sgRNA
Plasmid#190597PurposeThe plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.DepositorInsertsCas9
sgRNA
UseCRISPRTagsEGFP - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb-Cas9-mCherry_cU6:6-sgRNA
Plasmid#190598PurposeThe plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoterDepositorInsertsCas9
sgRNA
UseCRISPRTagsmCherry - separated by T2APromoterAedes aegypti polyubiquitin and Culex quinquefasc…Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#78231PurposeExpresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX165-HiFi Cas9
Plasmid#140563PurposeExpresses HiFi Cas9 in mammalian cellsDepositorInsertCas9
MutationR691AAvailable SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only