We narrowed to 7,945 results for: Lif;

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pGEMHE-mEGFP-Map4

Plasmid

#105571

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

MAP4 (Map4 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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RBD-Fc

Plasmid

#164221

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

Spike RBD (S SARS-CoV)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Giantin

Plasmid

#186575

Purpose

Expression of FRB-mCherry on Golgi membrane with Giantin transmembrane (residues 3131-3259)

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

Giantin (GOLGB1 Human)

Tags

FRB-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR2-tdTomato

Plasmid

#124236

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR2-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN-mCherry-Parkin

Plasmid

#59419

Purpose

to make mCherry-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

mCherry-Parkin (PRKN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pDM1383

Plasmid

#207423

Purpose

Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
Raf1 (RAF1 Human)

Use

Unspecified

Tags

GFP and mCherry

Mutation

aa 1–134 (RBD) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21ΔRING-Box

Plasmid

#105517

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of RING domain (aa124-462)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21ΔPRYSPRY

Plasmid

#105518

Purpose

Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

Plasmid

#127362

Purpose

gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ heiCas9

Plasmid

#186929

Purpose

For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

Depositor

Joachim Wittbrodt

Article

Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

Insert

myc-flexible linker-oNLS-Cas9-oNLS

Use

CRISPR

Tags

myc-flexible linker-optimizedNLS and optimizedNLS

Expression

Mammalian

Promoter

sp6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDL023_Tub-rb_IgG_HC

Plasmid

#193620

Purpose

α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-Tubulin scFv heavy variable + rabbit constant chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-DIO-hChR2(H134R)-P2A-EYFP

Plasmid

#139283

Purpose

Express hChR2(H134R)-P2A-EYFP in a Cre-dependent manner

Depositor

Mingshan Xue

Article

Chen et al Elife. 2020 Feb 19;9. pii: 48705. doi: 1

Insert

hChR2(H134R)-P2A-EYFP

Use

AAV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMIA4.721-Pansio

Plasmid

#214441

Purpose

BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

2xFKBP12-Casp9

Use

Hdr donor

Tags

P2A-Bsd

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2A

Plasmid

#115330

Purpose

expresses mCherry-CHMP2A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2A (CHMP2A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDL024_Tub-rb_IgG_LC

Plasmid

#193621

Purpose

α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-Tubulin scFv light variable + rabbit constant chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-FLAG-APEX2-MAP1LC3B

Plasmid

#175753

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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