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shha-pCRII

Plasmid

#68962

Purpose

for antisense probe, NotI w/ Sp6

Depositor

David Traver

Article

Clements et al Nature. 2011 Jun 8;474(7350):220-4. doi:

Insert

shha (shha Zebrafish)

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2U

Plasmid

#15810

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2U (UBE2U Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pJZC533

Plasmid

#135435

Purpose

Yeast integrating plasmid for pTEF1-mCherry reporter gene

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

mCherry

Expression

Yeast

Mutation

codon optimized for S. cerevisiae

Promoter

pTEF1

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHelper_TetR/pTet-Tnpase (carbR)

Plasmid

#213911

Purpose

ATc-inducible himar1C9 transposase expression - carb/amp resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

TetR/ptet ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[sfGFP]

Plasmid

#213902

Purpose

Mariner transposon expressing sfGFP with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and superfolder GFP

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Control-miR-17-5p-Sensor

Plasmid

#21166

Depositor

Joshua Mendell

Article

O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

Type

Empty backbone

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pAC1806_pmax-FKBP-dCasRx

Plasmid

#118639

Purpose

Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FKBP-dCasRx

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHR Fyn-TagBFP

Plasmid

#171035

Purpose

TagBFP-tagged Fyn kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Fyn

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1813_pCR8-gSMN2-DN1

Plasmid

#118646

Purpose

Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16

Plasmid

#51426

Purpose

Artificial transcription factor with VP16

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAC1823_pCR8-RBFOX1N-dCasRx-C

Plasmid

#118657

Purpose

Gateway Donor; RBFOX1N-dCasRx-C ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-dCasRx-C

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-Fn-crRNA

Plasmid

#78958

Purpose

Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP713

Plasmid

#45465

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP713

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-TRE-dCas9-EGFP

Plasmid

#62347

Purpose

Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

dCas9-EGFP

Use

CRISPR

Tags

HA and SV40 NLS

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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FLIParaF.Ec-250n

Plasmid

#18855

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

arabinose FRET sensor

Tags

CFP and Venus

Expression

Bacterial

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pRetroSuper Smad4

Plasmid

#15727

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad4 shRNA (SMAD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1C-shSCR

Plasmid

#139472

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1814_pCR8-gSMN2-DN2

Plasmid

#118647

Purpose

Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only