We narrowed to 20,061 results for: INO

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pGEMHE:mTREK-1(G171F)

Plasmid

#224747

Purpose

In vitro transcription for Oocyte expression of mTREK-1(G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

G171F

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK(S93C)

Plasmid

#224766

Purpose

In vitro transcription for Oocyte expression of mTRAAK(S93C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S93C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/K271Q)

Plasmid

#224750

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / K271Q

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(A286F)

Plasmid

#224746

Purpose

In vitro transcription for Oocyte expression of mTREK-1(A286F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

A286F

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem wt-S131C)

Plasmid

#224759

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S562C

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/G171F)

Plasmid

#224749

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / G171F

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-wt)

Plasmid

#224758

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/A286F)

Plasmid

#224748

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / A286F

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G137I)

Plasmid

#133272

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Use

Oocyte expression

Mutation

G137I

Promoter

T7

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRAW-457

Plasmid

#200213

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 H25A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-458

Plasmid

#200214

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 E184A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-459

Plasmid

#200215

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 K214A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-CI

Plasmid

#196849

Purpose

Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 hU6 ACTB sgRNA

Plasmid

#206270

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

ACTB sgRNA

Use

CRISPR; Multimate/gateway entr 2

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 hU6 HEK3 PegRNA

Plasmid

#206277

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

HEK3 PegRNA

Use

CRISPR; Multimate/gateway entr 2

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 3 polH aeBlue polH VSV-G

Plasmid

#206278

Purpose

ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

aeBlue, VSV-G

Use

Multimate/gateway entr 3

Expression

Bacterial and Insect

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

Plasmid

#196877

Purpose

Neuron-specific expression of mOrange2 reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-td-mCherry-CI

Plasmid

#196851

Purpose

Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

td-mCherry

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-ε ear

Plasmid

#196670

Purpose

Expresses GST-AP-4 ε ear (residues 840-1137) in bacteria

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε ear (840-1137) (AP4E1 Human)

Tags

GST

Expression

Bacterial

Mutation

silent mutations in codons 905, 1129 and 1137

Promoter

tac

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-TSF-ε

Plasmid

#196583

Purpose

Expresses the two Strep/one FLAG (TSF)-tagged human AP-4 ε subunit in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

Insert

AP-4 ε subunit (AP4E1 Human)

Tags

two Strep/one FLAG (TSF)

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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