We narrowed to 8,219 results for: Lif

Showing: 2201 - 2220 of 8219 results

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PyronicSF-mRuby2/pBI-CMV1

Plasmid

#124830

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Inserts

PyronicSF
mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21ΔPRYSPRY

Plasmid

#105524

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Mutation

deletion of PRYSPRY domain (aa1-281)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mEGFP-CHMP2A

Plasmid

#115328

Purpose

expresses mEGFP-CHMP2A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2A (CHMP2A Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Plasmid

#175777

Purpose

pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TMEM192 (TMEM192 Human)

Use

CRISPR

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-L2

Plasmid

#127350

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ heiCas9

Plasmid

#186929

Purpose

For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

Depositor

Joachim Wittbrodt

Article

Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

Insert

myc-flexible linker-oNLS-Cas9-oNLS

Use

CRISPR

Tags

myc-flexible linker-optimizedNLS and optimizedNLS

Expression

Mammalian

Promoter

sp6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR1-tdTomato

Plasmid

#124235

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR1-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:GtACR2-tdTomato

Plasmid

#124236

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR2-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Sema3e(L)-AP-His

Plasmid

#72019

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDL025_HA-rb_IgG_HC_15F11

Plasmid

#193622

Purpose

HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti_HA_15F11 rabbit heavy chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS-mCherry-Rim1[20-227]-mCherry

Plasmid

#246282

Purpose

Rab10 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Rim1[20-227]-mCherry (Rims1 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-Rims2[27-179]-mCherry

Plasmid

#246271

Purpose

Rab8 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Rims2[27-179]-mCherry (Rims2 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mCherry-Rabenosyn5[439-503]-mCherry

Plasmid

#246251

Purpose

Rab4 sensor acceptor

Depositor

Ryohei Yasuda

Article

Wang et al Elife. 2025 Sep 23;13:RP103879. doi: 10.

Insert

mCherry-Rabenosyn5[439-503]-mCherry (Zfyve20 Rat)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBABEpuro mTurquoise2 LC3B

Plasmid

#78518

Purpose

Fluorescent reporter for LC3B visualization

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

LC3B (MAP1LC3B Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Promoter

retroviral

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFE-Selo-FI-prk

Plasmid

#162698

Purpose

Expresses S. elongatus Form IB rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

S. elongatus Form IB rubisco and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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