We narrowed to 14,473 results for: shi

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pCAG-mSpata33 (I83A)-PA

Plasmid

#179132

Purpose

Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (Spata33 Mouse)

Tags

PA

Expression

Mammalian

Mutation

Changed Isoleucine 83 to Alanine

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

Plasmid

#178870

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA:Asip

Plasmid

#60968

Purpose

Expression vector of rat Asip guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

Agouti signaling protein gRNA (Asip Rat)

Use

CRISPR

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pT7-gRNA:kit-1

Plasmid

#60969

Purpose

Expression vector of rat Kit-1 guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)

Use

CRISPR

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pT7-gRNA:kit-2-1

Plasmid

#60970

Purpose

Expression vector of rat Kit-2-1 guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)

Use

CRISPR

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pT7-gRNA:kit-2-2

Plasmid

#60971

Purpose

Expression vector of rat Kit-2-2 guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)

Use

CRISPR

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMRX-IP-TMEM192-mKeima

Plasmid

#215358

Purpose

Probe to evaluate lysophagic activity

Depositor

Tamotsu Yoshimori

Article

Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

Insert

transmembrane protein 192 (TMEM192 Human)

Use

Retroviral

Tags

mKeima

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPlatTET-gRNA2

Plasmid

#82559

Purpose

All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

Depositor

Izuho Hatada

Article

Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

Insert

dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD

Expression

Mammalian

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-GFP

Plasmid

#129092

Purpose

KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

EGFP and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-TMEM192-mKeima

Plasmid

#215359

Purpose

Probe to evaluate lysophagic activity

Depositor

Tamotsu Yoshimori

Article

Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

Insert

transmembrane protein 192 (TMEM192 Human)

Use

Retroviral

Tags

mKeima

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3CB-FLAG

Plasmid

#179135

Purpose

Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CRX-T2A-NeuroD1

Plasmid

#194607

Purpose

Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

Depositor

Haruhisa Inoue

Article

Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

Insert

CRX-T2A-NeuroD1

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-CreER

Plasmid

#129093

Purpose

KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

CreERT2 and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-ACEmax

Plasmid

#139105

Purpose

Mammalian Target-ACEmax expression vector (pSI-625)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-LC3

Plasmid

#221738

Purpose

Stable expression of mRuby3-LC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LC3B (Map1lc3b Rat)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IZU-LAMP1-mRuby3

Plasmid

#222586

Purpose

Stable expression of LAMP1-mRuby3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

LAMP1 (LAMP1 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-Spo20(PABD)

Plasmid

#221758

Purpose

Stable expression of GFP-Spo20(PABD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Spo20 (SOS1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem Spo20(PABD) (amino acids 422-551)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-neo-hApg5(K130R)-HA

Plasmid

#22949

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Tags

3xHA

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pXPR_023d-sgCiCh2-2

Plasmid

#202444

Purpose

Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

Chromosome 2 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY-Rhotekin(8-89)-P2A-Clover(T153M/F223R)-RhoA

Plasmid

#165435

Purpose

Expresses ShadowY-Rhotekin(8-89) and Clover(T153M/F223R)-RhoA in mammalian cells to monitor Cdc42 activity with FLIM imaging.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

ShadowY-RBD-P2A-Clover(T153M/F223R)-RhoA

Tags

ShadowY, Clover(T153M/F223R)

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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