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pRB897

Plasmid

#124587

Purpose

C-terminal fusions of C. albicans optimized mScarlet fluorescent protein.

Depositor

Richard Bennett

Article

Frazer et al Curr Protoc Microbiol. 2019 Feb 12:e76.

Type

Empty backbone

Use

Synthetic Biology; Candida albicans expression

Tags

mScarlet

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512g3.1

Plasmid

#171200

Purpose

Expresses Br512g3.1 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLRD01

Plasmid

#208257

Purpose

Production of terpentetriene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cyc1
cyc2

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor1

Plasmid

#49352

Purpose

Promoterless luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

None

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+ABCB1a

Plasmid

#38036

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Promoter

SP6

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-HcRed

Plasmid

#11152

Purpose

Mammalian expression vector for expression of HcRed

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

HcRed1 from Heteractis crispa

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13

Plasmid

#110500

Purpose

Fluorescent Rab13

Depositor

Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab13 (RAB13 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pClusterin-ERT2CreERT2

Plasmid

#14796

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pROSA26-5'

Plasmid

#21715

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

ROSA26-5' insert (Gt(ROSA)26Sor Mouse)

Use

For use with mouse targeting vector prosa26-1

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pKAR2-Br512g3.2

Plasmid

#171201

Purpose

Expresses Br512g3.2 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKTol2C-EGFP

Plasmid

#85598

Purpose

Tol2 transposon with EGFP driven by minimal-CAGs promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with EGFP

Expression

Mammalian

Promoter

mini-CAG

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCR3.1-Luc

Plasmid

#106457

Purpose

luciferase reporter, CMV promoter

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

luciferase reporter, CMV promoter

Use

Luciferase

Expression

Mammalian

Mutation

WT promoter

Available Since

Nov. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR29b

Plasmid

#21121

Depositor

Joshua Mendell

Article

Hwang et al Science. 2007 Jan 5. 315(5808):97-100.

Insert

miR-29b-1 (MIR29B1 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
TMEM165 g2 LentiCRISPRv2-mCherry

Plasmid

#218661

Purpose

Knockout vector for human TMEM165

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM165 (TMEM165 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHA-N1

Plasmid

#86049

Purpose

A mammalian expression vector with a HA-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pROSA26-SAT

Plasmid

#208339

Purpose

ROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT signaling

Depositor

Philippe Soriano

Article

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Insert

SAT sensor

Use

Mouse Targeting

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-30uDelta13V

Plasmid

#18015

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

mglB-D154A

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Hbxadr-Hatag

Plasmid

#24930

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pCAG:ECFP

Plasmid

#32597

Depositor

Anna-Katerina Hadjantonakis

Article

Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

Insert

ECFP

Expression

Mammalian

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pQUAST-frt-stop-frt-p65AD::CaM

Plasmid

#64716

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Inserts

p65AD::CaM
frt-stop-frt

Expression

Insect

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pBa.NgCam.GFP

Plasmid

#45061

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

NgCam (L1CAM Chicken)

Tags

eGFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pPAS-E

Plasmid

#39867

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Insert

iRFP protein PAS domain

Tags

E coil

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pHZ081

Plasmid

#27506

Depositor

Andrew Fire

Article

Zhang et al Dev Biol. 2010 Aug 15. 344(2):603-10.

Insert

pcol-10::lin-4::let-858_3'UTR (lin-4 Nematode)

Expression

Worm

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2-bNWASP

Plasmid

#33019

Depositor

Marc Kirschner

Article

Lee et al Science. 2010 Sep 10;329(5997):1341-5.

Insert

N-WASP (WASL Bovine)

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mGAT1

Plasmid

#22804

Depositor

Henry Lester

Article

Mager et al J Neurosci. 1996 Sep 1. 16(17):5405-14.

Insert

pcDNA3.1-mGAT1

Expression

Mammalian

Mutation

wt

Available Since

Jan. 14, 2010

Availability

Academic Institutions and Nonprofits only

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Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)

Plasmid

#81252

Purpose

Mutation based toggling reporter (MTBR)

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Mutation Based Toggling Reporter (MBTR)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-QS

Plasmid

#24340

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QS

Tags

His and Myc

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG:EYFP

Plasmid

#32596

Depositor

Anna-Katerina Hadjantonakis

Article

Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

Insert

EYFP

Expression

Mammalian

Promoter

pCAGGs

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only